Comparing diversity patterns and processes of microbial community assembly in water column and sediment in Lake Wuchang, China

Xuemei Li; Zihao Meng; Kang Chen; Feifei Hu; Lu Liu; Tingbing Zhu; Deguo Yang

doi:10.7717/peerj.14592

Comparing diversity patterns and processes of microbial community assembly in water column and sediment in Lake Wuchang, China

PeerJ. 2023 Jan 5:11:e14592. doi: 10.7717/peerj.14592. eCollection 2023.

Authors

Xuemei Li¹, Zihao Meng¹, Kang Chen¹, Feifei Hu¹, Lu Liu¹, Tingbing Zhu¹, Deguo Yang¹

Affiliation

¹ Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Wuhan, China.

Abstract

The study compare the diversity patterns and processes of microbial community assembly in the water and sediment of Lake Wuchang (China) using high-throughput sequencing of 16S rRNA gene amplicons. A higher microbial α-diversity in the sediment was revealed (P < 0.01), and the most common bacterial phyla in water column were Proteobacteria, Cyanobacteria and Actinobacteria, while Proteobacteria, Acidobacteria, Chloroflexi and Nitrospirae were dominant in sediment. Functions related to phototrophy and nitrogen metabolism primarily occurred in the water column and sediment, respectively. The microbial communities in water column from different seasons were divided into three groups, while no such dispersion in sediment based on PCoA and ANOSIM. According to Pearson correlation analysis, water temperature, dissolved oxygen, water depth, total nitrogen, ammonium, and nitrite were key factors in determining microbial community structure in water column, while TN in sediment, conductivity, and organic matter were key factors in sediment. However, the stochastic processes (|βNTI| < 2) dominated community assembly in both the water column and sediment of Lake Wuchang. These data will provide a foundation for microbial development and utilization in lake water column and sediment under the circumstances of increasing tendency of lake ecological fishery in China.

Keywords: Environmental factors; Functional diversity; Lake Wuchang; Microbial community assembly; Species diversity; Stochastic processes.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cyanobacteria* / genetics
Lakes / microbiology
Microbiota* / genetics
Nitrogen
RNA, Ribosomal, 16S / genetics
Water

Substances

Water
RNA, Ribosomal, 16S
Nitrogen

Grants and funding

This study was funded by the National Key R&D Program of China (2019YFD090 0603), the China Agriculture Research System of MOF and MARA (CARS-46), and the Central Public-interest Scientific Institution Basal Research Fund, CAFS (2020XT1302, 2020TD57). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.