Background: Kadaknath is an important indigenous chicken with black pigmentation and cryopreserved semen reputably had low fertility.
Objective: The aim of this study was to evaluate the effects of betaine and raffinose in semen extenders on post thaw semen parameters and fertility.
Materials and methods: Semen was cryopreserved in 4% dimethyl sulfoxide (DMSO) with betaine supplemented at 0.1, 0.2 and 0.4 M or raffinose supplemented at 1, 5 and 10 mM. Post thaw semen parameters and fertility were evaluated.
Results: Betaine at higher concentrations significantly (p < 0.05) inhibited the post thaw sperm motility, live sperm and MTT dye reduction and a declining trend in the fertility with increasing betaine. Inclusion of raffinose had no effect on the post thaw in vitro semen parameters, however, the fertility was significantly (p < 0.05) higher in the 10 mM raffinose supplemented group.
Conclusion: Betaine has negative effect on post thaw semen parameters and raffinose at 10 mM concentration improves the fertility from cryopreserved semen. doi.org/10.54680/fr22510110212.