In-vitro anti-bacterial activity of medicinal plants against Urinary Tract Infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics

Mrityunjoy Acharjee; Nagma Zerin; Touhida Ishma; Md Rayhan Mahmud

doi:10.1016/j.nmni.2022.101076

In-vitro anti-bacterial activity of medicinal plants against Urinary Tract Infection (UTI) causing bacteria along with their synergistic effects with commercially available antibiotics

New Microbes New Infect. 2022 Dec 24:51:101076. doi: 10.1016/j.nmni.2022.101076. eCollection 2023 Jan.

Authors

Mrityunjoy Acharjee¹, Nagma Zerin¹, Touhida Ishma¹, Md Rayhan Mahmud²

Affiliations

¹ Department of Microbiology, Stamford University Bangladesh, 51 Siddeswari Road, Dhaka, 1217, Bangladesh.
² Department of Microbiology, Jagannath University, 9-10 Chittaranjan Ave, Dhaka, 1100, Bangladesh.

Abstract

Background: Plants contain a variety of bioactive compounds that provide them antimicrobial properties, which can be used to develop novel antibiotics. The current research evaluated the antibacterial activity of 6 medicinal plants Sphagneticola calendulacea (Chinese wedelia), Enydra fluctuans (Buffalo spinach), Chenopodium album (Goosefoot), Mentha arvensis (Wild mint), Mimosa diplotricha (Nila grass), and Averrhoa bilimbi (Cucumber tree) against Urinary Tract Infection (UTI)- causing pathogens (Staphylococcus spp., Proteus spp., Pseudmonas spp., Escherichia coli and Enterobacter spp.).

Methods: The bacterial contamination of these plants was evaluated by using their surface-washed water. The combined effects of commercially available antibiotics along with these medicinal plants were also tested. We used the solvent extraction method, conventional cell culture technique, minimum inhibitory concentration (MIC) assay, and disc diffusion method for our analysis.

Results: The surface-washed water was contaminated with variable bacteria. The plants displayed notable antibacterial activity against most of the tested bacteria. Ethanol and hot water extract of plants exhibited minimum inhibitory effects, while the methanol extract of plants showed very potent antibacterial activity against most of the bacteria with inhibitory zone diameter up to 14 mm. In the case of combined effects, the zone diameter increased up to 26 mm, which is a significant improvement compared to the individual plant extracts.

Interpretation: This data suggested that the combination of two antibacterial agents, one natural and the other synthetic, would be more efficient in the treatment of multidrug-resistant bacteria than a single monotherapy of either of the antibacterial agents.

Keywords: Antibacterial activity; Medicinal Plants; Synergistic effects; UTI causing-pathogens.