Theranostic doxorubicin encapsulated FeAu alloy@metal-organic framework nanostructures enable magnetic hyperthermia and medical imaging in oral carcinoma

Udesh Dhawan; Ching-Li Tseng; Ping-Hsuan Wu; Mei-Yi Liao; Huey-Yuan Wang; Kevin C-W Wu; Ren-Jei Chung

doi:10.1016/j.nano.2023.102652

Theranostic doxorubicin encapsulated FeAu alloy@metal-organic framework nanostructures enable magnetic hyperthermia and medical imaging in oral carcinoma

Nanomedicine. 2023 Feb:48:102652. doi: 10.1016/j.nano.2023.102652. Epub 2023 Jan 7.

Authors

Udesh Dhawan¹, Ching-Li Tseng², Ping-Hsuan Wu³, Mei-Yi Liao⁴, Huey-Yuan Wang⁵, Kevin C-W Wu⁶, Ren-Jei Chung⁷

Affiliations

¹ Centre for the Cellular Microenvironment, Division of Biomedical Engineering, School of Engineering, University of Glasgow, Glasgow G12 8LT, UK.
² Graduate Institute of Biomedical Materials and Tissue Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 110, Taiwan; International Ph. D. Program in Biomedical Engineering, College of Biomedical Engineering, Taipei Medical University, Taipei City 110, Taiwan; Research Center of Biomedical Device, College of Biomedical Engineering, Taipei Medical University, Taipei City 110, Taiwan; International Ph. D. Program in Cell Therapy and Regenerative Medicine, College of Medicine, Taipei Medical University, Taipei City 110, Taiwan.
³ Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei 106344, Taiwan.
⁴ Department of Applied Chemistry, National Pingtung University, Pingtung 90003, Taiwan.
⁵ Department of Stomatology, MacKay Memorial Hospital, Taipei 104217, Taiwan. Electronic address: wang461110@yahoo.com.tw.
⁶ Department of Chemical Engineering, National Taiwan University, Taipei 10617, Taiwan; Institute of Biomedical Engineering & Nanomedicine, National Health Research Institute, Keyan Road, Zhunan, Miaoli City 350, Taiwan; Center of Atomic Initiative for New Materials (Al-MAT), National Taiwan University, Taipei 106216, Taiwan. Electronic address: kevinwu@ntu.edu.tw.
⁷ Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei 106344, Taiwan. Electronic address: rjchung@mail.ntut.edu.tw.

PMID: 36623714
DOI: 10.1016/j.nano.2023.102652

Abstract

Metal-organic frameworks (MOFs) have emerged as attractive candidates in cancer theranostics due to their ability to envelop magnetic nanoparticles, resulting in reduced cytotoxicity and high porosity, enabling chemodrug encapsulation. Here, FeAu alloy nanoparticles (FeAu NPs) are synthesized and coated with MIL-100(Fe) MOFs to fabricate FeAu@MOF nanostructures. We encapsulated Doxorubicin within the nanostructures and evaluated the suitability of this platform for medical imaging and cancer theranostics. FeAu@MOF nanostructures (FeAu@MIL-100(Fe)) exhibited superparamagnetism, magnetic hyperthermia behavior and displayed DOX encapsulation and release efficiency of 69.95 % and 97.19 %, respectively, when stimulated with alternating magnetic field (AMF). In-vitro experiments showed that AMF-induced hyperthermia resulted in 90 % HSC-3 oral squamous carcinoma cell death, indicating application in cancer theranostics. Finally, in an in-vivo mouse model, FeAu@MOF nanostructures improved image contrast, reduced tumor volume by 30-fold and tumor weight by 10-fold, which translated to enhancement in cumulative survival, highlighting the prospect of this platform for oral cancer treatment.

Keywords: Hyperthermia; Metal-organic framework; Oral squamous carcinoma; cancer theranostics; iron‑gold alloy nanoparticles.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Carcinoma*
Diagnostic Imaging
Doxorubicin / chemistry
Doxorubicin / pharmacology
Doxorubicin / therapeutic use
Hyperthermia, Induced*
Magnetic Phenomena
Metal-Organic Frameworks* / chemistry
Mice
Mouth Neoplasms* / diagnostic imaging
Mouth Neoplasms* / drug therapy
Nanostructures*
Precision Medicine
Theranostic Nanomedicine

Substances

Metal-Organic Frameworks
Doxorubicin