Analysis of photosynthetic pigments pathway produced by CO2-toxicity-induced Scenedesmus obliquus

Abstract

The coal-to-gas process produces carbon dioxide, which increases global warming, and its wastewater treatment generates sludge with high organic toxicity. Scenedesmus obliquus is a potential solution to such environmental problems, and photosynthetic pigments are the focus of this study. The optimal concentration of CO₂ for the growth of Scenedesmus obliquus was found to be 30 % after increasing the concentration of CO₂ (0.05 %-100 %). The accumulation of photosynthetic pigments during cultivation could reach 31.74 ± 1.33 mg/L, 11.21 ± 0.42 mg/L, and 5.59 ± 0.19 mg/L respectively, and the organic toxicity of sludge extract could be reduced by 44.97 %. Upregulation of A0A383VSL5, A0A383WMQ3, and A0A2Z4THB7 as photo systemic oxygen release proteins and propylene phosphate isomerase resulted in oxygen-evolving proteins in photosystem II, electron transport in photosystem I, and intermediates in carbon fixation. This is achieved by increasing the intracellular antennae protein and carbon fixation pathway, allowing Scenedesmus obliquus to both tolerate and fix CO₂ and reduce the organic toxicity of sludge. These findings provide insights into the innovative strategy underlining the fixation of CO₂, treatment and disposal of industrial residual sludge, and the enhancement of microalgal biomass production.

Keywords: CO(2) bio-fixation; Photosynthetic pigments; Proteomics analysis; Scenedesmus obliquus; Sludge organic toxicity reduction.