Mitochondria-targeted fluorescent probe with long wavelength emission for detecting H2S and its application in foodstuff, water and living cells

Ling Xie; Renjie Wang; Congbin Fan; Yayi Tu; Gang Liu; Shouzhi Pu

doi:10.1016/j.foodchem.2023.135411

Mitochondria-targeted fluorescent probe with long wavelength emission for detecting H₂S and its application in foodstuff, water and living cells

Food Chem. 2023 Jun 1:410:135411. doi: 10.1016/j.foodchem.2023.135411. Epub 2023 Jan 5.

Authors

Ling Xie¹, Renjie Wang¹, Congbin Fan², Yayi Tu¹, Gang Liu¹, Shouzhi Pu³

Affiliations

¹ Jiangxi Key Laboratory of Organic Chemistry, College of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330031, PR China.
² Jiangxi Key Laboratory of Organic Chemistry, College of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330031, PR China. Electronic address: congbinfan@163.com.
³ Jiangxi Key Laboratory of Organic Chemistry, College of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University, Nanchang 330031, PR China; Department of Ecology and Environment, Yuzhang Normal University, Nanchang 330103, PR China. Electronic address: pushouzhi@tsinghua.org.cn.

PMID: 36623459
DOI: 10.1016/j.foodchem.2023.135411

Abstract

Hydrogen sulfide (H₂S) is crucial to cellular energy production, apoptosis, and redox homeostasis in mitochondria of living cells. In this work, a unique mitochondria-targeting fluorescence probe (DDMI) was established for H₂S determination based on styrylpyridinium scaffold. When DDMI was treated with H₂S, it showed significant fluorescence enhancement at 623 nm, with good selectivity, and high sensitivity. In addition, the "turn-on" fluorescent probe DDMI could detect H₂S in water samples with good recoveries in the range of 95.4 %-105.6 % and track the degree of food spoilage by visualizing the change of DDMI-loaded test strips. Furthermore, the established probe DDMI was successfully used for monitoring exogenous H₂S in living cells and mitochondria targeting. These results paved the way for success in developing a technology that could be used to identify H₂S in environment, foodstuff, and living cells.

Keywords: Foodstuff; Hydrogen sulfide; Living cells imaging; Long wavelength emission; Mitochondria targeting.

MeSH terms

Fluorescent Dyes*
HeLa Cells
Humans
Hydrogen Sulfide*
Mitochondria
Spectrometry, Fluorescence
Water

Substances

Fluorescent Dyes
Water
Hydrogen Sulfide