Stability of glycosylated complexes loaded with Epigallocatechin 3-gallate (EGCG)

Jianyong Zhang; Hongchun Cui; Jiahuan Qiu; Xiaoqing Wang; Yixin Zhong; Caiping Yao; Lanying Yao; Qunxiong Zheng; ChunHua Xiong

doi:10.1016/j.foodchem.2022.135364

Stability of glycosylated complexes loaded with Epigallocatechin 3-gallate (EGCG)

Food Chem. 2023 Jun 1:410:135364. doi: 10.1016/j.foodchem.2022.135364. Epub 2022 Dec 29.

Authors

Jianyong Zhang¹, Hongchun Cui², Jiahuan Qiu³, Xiaoqing Wang³, Yixin Zhong³, Caiping Yao³, Lanying Yao³, Qunxiong Zheng³, ChunHua Xiong⁴

Affiliations

¹ Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou 310012, PR China; Tea Research Institute, Chinese Academy of Agricultural Science, Hangzhou 310008, PR China.
² Tea Research Institute, Hangzhou Academy of Agricultural Science, Hangzhou 310024, PR China.
³ Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou 310012, PR China.
⁴ Department of Applied Chemistry, Zhejiang Gongshang University, Hangzhou 310012, PR China. Electronic address: xiongch@163.com.

PMID: 36623458
DOI: 10.1016/j.foodchem.2022.135364

Abstract

The application of Epigallocatechin-3-gallate (EGCG) in food industry was limited by its low stability in aqueous solutions and poor bioavailability in vivo. The novel EGCG glycosylated arachin nanoparticles (Ara-CMCS-EGCG) and EGCG glycosylated casein nanoparticles (Cas-CMCS-EGCG) were prepared to improve the stability and bioavailability of EGCG. The effect of different variables on the storage stability and the slow-release behavior of novel glycosylation complexes in nanoparticle background solution and artificial gastrointestinal fluid were investigated. The results showed that the DPPH scavenging activity of Ara-CMCS-EGCG and Cas-CMCS-EGCG were stable in temperature (25 ∼ 70 °C). EGCG could enhance the crosslinking effect of molecular particles in glycosylation complexes solution. The glycosylated protein nanoparticles were stable to acid-base and enzymolysis in simulated gastrointestinal fluid. The release rate of EGCG in simulated intestinal fluid was higher than that in simulated gastric fluid. The glycosylated protein carrier can not only release EGCG slowly, but also significantly improve the stability and bioavailability of EGCG in simulated gastrointestinal fluid.

Keywords: Antioxidation; Arachin; Casein; EGCG; Glycosylation; Stability.

MeSH terms

Catechin* / chemistry
Drug Stability
Glycoproteins
Glycosylation
Nanoparticles* / chemistry

Substances

gallocatechol
epigallocatechin gallate
Catechin
Glycoproteins