Simultaneous screening of multiple diarrhetic shellfish poisons with group-specific split aptamers and silver nanocluster beacon

Lele Wang; Wenjing Wang; Ling Zhang; Jiao Li; Jianan Sun; Sai Wang; Xiangzhao Mao

doi:10.1016/j.foodchem.2023.135389

Simultaneous screening of multiple diarrhetic shellfish poisons with group-specific split aptamers and silver nanocluster beacon

Food Chem. 2023 Jun 1:410:135389. doi: 10.1016/j.foodchem.2023.135389. Epub 2023 Jan 3.

Authors

Lele Wang¹, Wenjing Wang¹, Ling Zhang¹, Jiao Li¹, Jianan Sun¹, Sai Wang², Xiangzhao Mao³

Affiliations

¹ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China.
² College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China. Electronic address: wangsai@ouc.edu.cn.
³ College of Food Science and Engineering, Ocean University of China, Qingdao 266003, China; Laboratory for Marine Drugs and Bioproducts of Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China.

PMID: 36623457
DOI: 10.1016/j.foodchem.2023.135389

Abstract

Poisoning events concerning diarrhetic shellfish poisons (DSPs) are increasing continually. It is extremely necessary to develop simple analysis methods for screening simultaneously different types of DSPs from food-related samples. Okadaic acid (OA) and its analogues, i.e., dinophysistoxin-1 (DTX-1) and dinophysistoxin-2 (DTX-2), are the prevalent DSPs. Herein, a facile and label-free fluorescent aptasensor targeting the three DSPs was constructed with a pair of group-specific split aptamers and silver nanocluster beacon. In presence of the targets, the DNA templates attached at the ends of the split aptamers would be dragged close to trigger enhanced fluorescence signals from silver nanoclusters. The aptasensor offered high sensitivity and good selectivity, with limit of detection of 2.282 nmolL^-1, 19.38 nmolL^-1, and 13.61 nmolL^-1 for OA, DTX-1, and DTX-2, respectively. Moreover, the applicability of aptasensor was well verified with shellfish and seawater samples. This study provides good reference for further exploration on analysis methods for food-related molecules.

Keywords: Diarrhetic shellfish poisons; Group-specific aptamer; Label-free aptasensor; Silver nanocluster beacon; Simultaneous screening.

MeSH terms

Humans
Marine Toxins / analysis
Okadaic Acid / analysis
Poisons* / analysis
Pyrans / analysis
Shellfish / analysis
Shellfish Poisoning*
Silver

Substances

dinophysistoxin 2
Marine Toxins
Silver
Pyrans
Okadaic Acid
dinophysistoxin 1
Poisons