An understanding of the rotary cycle of molecular motors (MMs), a key component of an approach to opening cells using mechanical motion, is important in furthering the research. Nuclear magnetic resonance (NMR) spectroscopy was used for in situ analysis of illuminated light-active MMs. We found that the presence of a N,N-dimethylethylenediamine in a position conjugated to the central olefin results in changes to the rotation of a second-generation Feringa-type MM. Importantly, the addition decreases the photostability of the compound. The parent compound 1 can withstand >2 h of illumination with no signs of decomposition, while the amino 7 decomposes after 10 min. We found that the degradation can be mitigated by implementing the simple techniques of modulating the light dose, dilution, and stirring the sample while illuminating. Additionally, the presence of moisture affects the rate of the motor's rotation. The addition of the amino group to 1, without moisture present, makes the rotation of motor 7 three times slower than the unfunctionalized parent compound. We also report the use of a method that can be used to determine the molar extinction coefficient of a light-generated metastable species. This method can be used when in situ NMR illumination is not available.