Sensitive and Selective Voltammetric Sensor Based on Anionic Surfactant-Modified Screen-Printed Carbon for the Quantitative Analysis of an Anticancer Active Fused Azaisocytosine-Containing Congener

Jędrzej Kozak; Katarzyna Tyszczuk-Rotko; Krzysztof Sztanke; Małgorzata Sztanke

doi:10.3390/ijms24010564

Sensitive and Selective Voltammetric Sensor Based on Anionic Surfactant-Modified Screen-Printed Carbon for the Quantitative Analysis of an Anticancer Active Fused Azaisocytosine-Containing Congener

Int J Mol Sci. 2022 Dec 29;24(1):564. doi: 10.3390/ijms24010564.

Authors

Jędrzej Kozak¹, Katarzyna Tyszczuk-Rotko¹, Krzysztof Sztanke², Małgorzata Sztanke³

Affiliations

¹ Faculty of Chemistry, Institute of Chemical Sciences, Maria Curie-Skłodowska University in Lublin, 20-031 Lublin, Poland.
² Laboratory of Bioorganic Compounds Synthesis and Analysis, Medical University of Lublin, 4A Chodźki Street, 20-093 Lublin, Poland.
³ Department of Medical Chemistry, Medical University of Lublin, 4A Chodźki Street, 20-093 Lublin, Poland.

Abstract

3-(4-Nitrophenyl)-8-(2,3-dimethylphenyl)-7,8-dihydroimidazo[2,1-c][1,2,4]triazin-4(6H)-one (NDIT) is one of the most promising candidates for anticancer agents. Hence, a sensitive and selective sodium dodecyl sulfate-modified screen-printed carbon sensor (SPCE/SDS) was used for its quantitative analysis. The SPCE/SDS, in contrast to the SPCE, showed excellent behavior in the electrochemical reduction of NDIT by differential-pulse adsorptive stripping voltammetry (DPAdSV). Cyclic voltammetric (CV) studies reveal an irreversible, two-stage and not purely diffusion-controlled reduction process in 0.01 M HNO₃. The sensor was characterized by CV and electrochemical impedance spectroscopy (EIS). Under the optimized conditions (t 45 s, ΔE 175 mV, ν 150 mV/s, and t_m 5 ms), the DPAdSV procedure with the SPCE/SDS presented a very wide linear range from 1 to 2000 nM and a low detection limit of 0.29 nM. A 1000-fold excess concentration of potential interferents commonly present in biological samples did not significantly alter the peak current of NDIT. The practical application of the proposed DPAdSV procedure with the SPCE/SDS was successfully checked by analyzing spiked human serum samples.

Keywords: anionic surfactant; anticancer agent candidate; fused azaisocytosine-containing congener with the para-nitrophenyl group; screen-printed sensor; voltammetry.

MeSH terms

Carbon* / chemistry
Electrochemical Techniques / methods
Electrodes
Humans
Sodium Dodecyl Sulfate
Surface-Active Agents*

Substances

Carbon
Surface-Active Agents
Sodium Dodecyl Sulfate

Grants and funding

This research received no external funding.