Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice

Vural Yilmaz; Katerina Strati

doi:10.1016/j.xpro.2022.101994

Protocol for in vivo lineage tracing of the mouse-papillomavirus-type 1-infected cells in mice

STAR Protoc. 2023 Mar 17;4(1):101994. doi: 10.1016/j.xpro.2022.101994. Epub 2023 Jan 4.

Authors

Vural Yilmaz¹, Katerina Strati²

Affiliations

¹ Department of Biological Sciences, University of Cyprus, Nicosia 2109, Cyprus.
² Department of Biological Sciences, University of Cyprus, Nicosia 2109, Cyprus. Electronic address: strati@ucy.ac.cy.

Abstract

Here, we present a protocol to create an in vivo lineage-tracing mouse model for mouse-papillomavirus-type 1 (MmuPV1)-infected cells. We describe the steps to generate and deliver the MmuPV1 lox-Cre-lox plasmid for the infection of mice, followed by skin tissue extraction and processing. We then detail how to use flow cytometry to trace, quantify, and analyze MmuPV1-harboring cells and their progeny. This model is suitable to investigate the early effects of papillomavirus on the target cells. For complete details on the use and execution of this protocol, please refer to Yilmaz et al. (2022).¹.

Keywords: Cell Biology; Flow Cytometry/Mass Cytometry; Microbiology; Model Organisms; Molecular Biology.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Flow Cytometry*
Mice
Papillomaviridae*
Papillomavirus Infections*