High-performance liquid chromatography using diode array detector and fluorescence detector for hydrogen peroxide analysis in processed fishery foods

Juhee Park; Heemin Noh; Hee-Jae Suh; Dojin Ryu; Hyun Jung Lee; Chan Lee

doi:10.1007/s10068-022-01165-1

High-performance liquid chromatography using diode array detector and fluorescence detector for hydrogen peroxide analysis in processed fishery foods

Food Sci Biotechnol. 2022 Sep 12;32(1):27-37. doi: 10.1007/s10068-022-01165-1. eCollection 2023 Jan.

Authors

Juhee Park^{1

2}, Heemin Noh¹, Hee-Jae Suh³, Dojin Ryu⁴, Hyun Jung Lee⁴, Chan Lee¹

Affiliations

¹ Department of Food Science and Biotechnology, Chung-Ang University, Anseong, Gyeonggi 17546 Republic of Korea.
² Food Analysis Research Center, Food Industry Research Division, Wanju, Jeollabuk-do 55365 Republic of Korea.
³ Department of Food Science, Sun Moon University, Asan, Chungchengnam-do 31460 Republic of Korea.
⁴ Department of Animal, Veterinary, and Food Sciences, University of Idaho, 875 Perimeter Drive MS 2330, Moscow, ID 83844-2330 USA.

Abstract

Hydrogen peroxide (H₂O₂) is a food additive for bleaching and sterilization, owing to its strong oxidizing effect. The current study aimed to develop analytical methods to detect trace amounts of residual H₂O₂ in diverse foods using high-performance liquid chromatography (HPLC) equipped with diode array detector (DAD) or fluorescence detector (FLD). The vanadium(V)-peroxo complex, derived from the reaction of H₂O₂ with ammonium metavanadate, was used for the detection of H₂O₂ with DAD. H₂O₂ was indirectly analyzed using FLD via the detection of 7-hydroxycoumarin, derived by Fenton reaction, followed by verification using liquid chromatography with tandem mass spectrometry analysis. Both the detection methods showed good linearity with R² > 0.997. Limit of detection and limit of quantification were 0.30 and 0.91 mg/L (8.82 and 26.76 μM) with HPLC-DAD and 0.001 and 0.003 mg/L (0.03 and 0.09 μM) with HPLC-FLD, respectively. Applicability of both the methods was successively tested through sample analysis.

Keywords: Derivative; HPLC–DAD; HPLC–FLD; Hydrogen peroxide; Method validation.

© The Korean Society of Food Science and Technology 2022, Springer Nature or its licensor holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.