Protective effect of fermented aloe extract on glutamate-induced cytotoxicity in HT22 cells

Ki Beom Jeon; Seong Hun Lee; Yong Seong Kwon; Jin Hong Beak; Hyeon Lee; Choong Je Ma

doi:10.1080/19768354.2022.2147584

Protective effect of fermented aloe extract on glutamate-induced cytotoxicity in HT22 cells

Anim Cells Syst (Seoul). 2022 Nov 22;26(6):318-327. doi: 10.1080/19768354.2022.2147584. eCollection 2022.

Authors

Ki Beom Jeon¹, Seong Hun Lee², Yong Seong Kwon², Jin Hong Beak², Hyeon Lee¹, Choong Je Ma^{3

4}

Affiliations

¹ R&DB Center, Beauty Science, Ltd., Sejong, Korea.
² R&D center, KJM Aloe Co., Ltd., Seoul, Korea.
³ Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon, South Korea.
⁴ Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, South Korea.

Abstract

Excessive glutamate can cause oxidative stress in neuronal cells and this can significantly contribute to the etiology of neurodegenerative disease. The present study mainly aims to investigate that aloe extract (AE) and fermented aloe extract (FAE) could protect against glutamate-induced cytotoxicity by modulating oxidative stress. In this study, both AE and FAE showed potent neuroprotective activity by inhibiting ROS and Ca²⁺ concentration, increasing mitochondria membrane potential, and activating glutathione-related enzymes against glutamate-insulted neurotoxicity in HT22 cells. In addition, the neuroprotective activity of FAE was more potent than that of AE. HPLC analysis reveals that the chemical composition of FAE is different from that of AE. Especially, the contents of aloin A, aloin B and aloenin were higher in FAE than in AE. In conclusion, this study indicates that both AE and FAE may have effective neuroprotective activity in glutamate-insulted pathological conditions such as Alzheimer's disease by managing oxidative stress.

Keywords: Aloe arborescens; aloenin; aloin; neuroprotection; oxidative stress.

Grants and funding

This research was financially supported by the Ministry of Small and Medium-sized Enterprises(SMEs) and Startups(MSS), Korea, under the ‘Regional Specialized Industry Development Plus Program(R&D, S3087870)’ supervised by the Korea Institute for Advancement of Technology(KIAT ).