Background: Dental caries remain a significant global health challenge. Unfortunately, current dental materials lack sufficient antimicrobial power to address the pathogenic species involved in this disease. In this study the potential to load a dental resin blend (RB) with riboflavin (B2) for use in an antimicrobial photodynamic therapy (aPDT) approach was investigated.
Methods: B2 was added to our experimental RB (0.1 - 10 wt%). Upon investigating the degree of conversion and specimen integrity of the RB as a function of B2 concentration, it was determined that loading should be restricted to 0.1, 1.0, and 1.5 wt%. Subsequent investigation included water sorption (WS) and solubility (SL), as well as shear bond strength (SBS) and flexural strength (FS) of the specimens after 24 h and 28-day water storage. Lastly, the antimicrobial response of Streptococcus mutans (S. mutans) biofilm following 6 h growth and 60 s of blue LED light (1.3 J/cm2) in an aPDT-based approach was measured.
Results and conclusions: Adding up to 1.5 wt% B2 had minimal impact on the FS or SBS of the RB. However, aging for 28-days notably increased the FS by as much as 50% for the 1.5 wt% B2-loaded RB. In addition, adding 1.5 wt% B2 resulted in a significant reduction in WS/SL of the RB. Lastly, while adding B2 did not change the antimicrobial response, this was an initial study under these conditions and future investigation will seek to optimize light parameters to produce a more agonistic response. Overall, a riboflavin-loaded dental resin shows significant promise for utilization in restorative dentistry with aPDT.
Keywords: Dental material; Dental resin; Methacrylate; Photodynamic therapy; Photosensitizer; Riboflavin.
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