Overexpressing GRE3 in Saccharomyces cerevisiae enables high ethanol production from different lignocellulose hydrolysates

Haijie Wang; Limin Cao; Qi Li; Nalin N Wijayawardene; Jian Zhao; Min Cheng; Qi-Rui Li; Xiaobin Li; Itthayakorn Promputtha; Ying-Qian Kang

doi:10.3389/fmicb.2022.1085114

Overexpressing GRE3 in Saccharomyces cerevisiae enables high ethanol production from different lignocellulose hydrolysates

Front Microbiol. 2022 Dec 19:13:1085114. doi: 10.3389/fmicb.2022.1085114. eCollection 2022.

Authors

Haijie Wang¹, Limin Cao², Qi Li², Nalin N Wijayawardene^{3

4

5}, Jian Zhao⁶, Min Cheng^{1

7}, Qi-Rui Li¹, Xiaobin Li⁸, Itthayakorn Promputtha^{9

10}, Ying-Qian Kang¹

Affiliations

¹ Key Laboratory of Medical Microbiology and Parasitology & Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, Guizhou, China.
² Beijing Key Laboratory of Plant Gene Resources and Biotechnology for Carbon Reduction and Environmental Improvement, College of Life Sciences, Capital Normal University, Beijing, China.
³ Center for Yunnan Plateau Biological Resources Protection and Utilization, College of Biological Resource and Food Engineering, Qujing Normal University, Qujing, China.
⁴ Section of Genetics, Institute for Research and Development in Health and Social Care, Battaramulla, Sri Lanka.
⁵ National Institute of Fundamental Studies, Kandy, Sri Lanka.
⁶ State key Laboratory of Microbial Technology, Shandong University, Qingdao, China.
⁷ Department of Hospital Infection Management, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou, China.
⁸ Chishui Riverside Jiangi-Flavour Baijiu Research Center, Guizhou Sunveen Liquor Co., Ltd, Guiyang, China.
⁹ Environmental Science Research Center, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.
¹⁰ Department of Biology, Faculty of Science, Chiang Mai University, Chiang Mai, Thailand.

Abstract

The efficiently renewable bioethanol can help to alleviate energy crisis and environmental pollution. Genetically modified strains for efficient use of xylose and developing lignocellulosic hydrolysates play an essential role in facilitating cellulosic ethanol production. Here we present a promising strain GRE3^OE via GRE3 overexpressed in a previously reported Saccharomyces cerevisiae strain WXY70. A comprehensive evaluation of the fermentation level of GRE3^OE in alkaline-distilled sweet sorghum bagasse, sorghum straw and xylose mother liquor hydrolysate. Under simulated corn stover hydrolysate, GRE3^OE produced 53.39 g/L ethanol within 48 h. GRE3^OE produced about 0.498 g/g total sugar in sorghum straw hydrolysate solution. Moreover, GRE3^OE consumed more xylose than WXY70 in the high-concentration xylose mother liquor. Taken together, GRE3^OE could be a candidate strain for industrial ethanol development, which is due to its remarkable fermentation efficiency during different lignocellulosic hydrolysates.

Keywords: GRE3; Saccharomyces cerevisiae; ethanol; lignocellulosic hydrolysates; xylose.