The search for systemic biomarkers for monitoring degenerative lumbar spinal disorders

Nader Tarabeih; Adel Shalata; Orabi Higla; Alexander Kalinkovich; Gregory Livshits

doi:10.1016/j.ocarto.2022.100323

The search for systemic biomarkers for monitoring degenerative lumbar spinal disorders

Osteoarthr Cartil Open. 2022 Nov 24;4(4):100323. doi: 10.1016/j.ocarto.2022.100323. eCollection 2022 Dec.

Authors

Nader Tarabeih^{1

2}, Adel Shalata³, Orabi Higla⁴, Alexander Kalinkovich¹, Gregory Livshits⁵

Affiliations

¹ Department of Anatomy and Anthropology, Sackler Faculty of Medicine, Tel-Aviv University, Tel-Aviv, Israel.
² Maale HaCarmel Mental Health Center, Affiliated to Rappaport Faculty of Medicine Technion, Israel Institute of Technology, Haifa, Israel.
³ The Simon Winter Institute for Human Genetics, Bnai Zion Medical Center, The Ruth and Bruce Rappaport Faculty of Medicine, Technion, Haifa, Israel.
⁴ Department of Orthopedic Surgery, Sourasky Medical Center, Tel Aviv, Israel.
⁵ Deparment of Morphological Sciences, Adelson School of Medicine, Ariel University, Ariel, Israel.

Abstract

Objectives: In our previous study, we reported that low back pain (LBP) severity and disability significantly correlate with body composition and several blood biochemical factors. Herein, we tested the hypothesis that these covariates are associated with anatomical deformations of the lumbar spine, in particular, radiographic facet joint osteoarthritis (FJOA) and lumbar disc degeneration (LDD) features important contributors to LBP.

Methods: CT and MRI images of the lumbar spine were obtained from 200 individuals suffering from LBP-sciatica. We examined the FJOA and total LDD score - the sum of the scores of the three radiographic features (intervertebral disc herniation, osteophythosis and spondylolisthesis) at the L1 - S1 vertebral levels. By implementing a bioelectrical impedance analysis, we assessed the participants for body composition, specifically, extracellular water (ECW). Plasma levels of growth and differentiation factor 15 (GDF-15) and visceral adipose tissue-derived serine protease inhibitor (vaspin), were detected by ELISA.

Results: By conducting a series of multivariable regression analyses, we report that the circulating levels of GDF-15, vaspin, and ECW are significantly and independently associated with FJOA scores [β_GDF15 = 0.38 ± 0.08, p = 0.0001; β_VASPIN = 0.36 ± 0.07, p = 0.000004; β_ECW = 0.24 ± 0.07, p = 0.002]. The levels of GDF-15 (β = 0.30 ± 0.10, p = 0.007) and ECW (β = 0.20 ± 0.09, p = 0.03) were also found significantly associated with the LDD scores.

Conclusion: The obtained new data suggest that GDF-15, vaspin and ECW may serve as biomarkers for FJOA and LDD phenotypes.

Keywords: Body composition; Facet joint osteoarthritis; GDF-15; Low back pain; Lumbar intervertebral disk; Vaspin.