Inhibition and Removal of Mature Mixed-Bacteria Biofilms on Voice Prostheses by Sodium Selenite

Yongli Zhang; Yanyan Niu; Hong Huo; Jian Wang; Xiaofeng Jin; Hua Yang

doi:10.2147/IDR.S393434

Inhibition and Removal of Mature Mixed-Bacteria Biofilms on Voice Prostheses by Sodium Selenite

Infect Drug Resist. 2022 Dec 29:15:7799-7810. doi: 10.2147/IDR.S393434. eCollection 2022.

Authors

Yongli Zhang^{1

2}, Yanyan Niu^{1

2}, Hong Huo^{1

2}, Jian Wang^{1

2}, Xiaofeng Jin^{1

2}, Hua Yang^{1

2}

Affiliations

¹ Peking Union Medical College, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Department of Otolaryngology, Beijing, People's Republic of China.
² Translational Medicine Center, Peking Union Medical College Hospital, Beijing, People's Republic of China.

Abstract

Purpose: Biofilms on voice prostheses are important factors shortening their service life. Sodium selenite has been used to prevent and treat various diseases. Whether sodium selenite can inhibit and remove mature biofilms on voice prostheses is still unknown.

Methods: To verify the effects of sodium selenite on mature mixed-bacteria biofilms (Staphylococcus aureus, Candida albicans, and Streptococcus faecalis) on voice prostheses, we used quantitative and qualitative methods, eg, real-time fluorescence quantitative PCR, crystal violet staining, 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide) (XTT) reduction assays, and scanning electron microscopy, to measure the effects of sodium selenite on the number of bacterial colonies, biofilm formation ability, metabolic activity, and ultrastructure in a model of mature mixed-bacteria biofilms on voice prostheses and validated the effects in vitro on mature biofilms on voice prostheses from patients.

Results: When exploring the possible mechanism of biofilm inhibition and removal by sodium selenite, we found that it significantly inhibited and removed biofilms on voice prostheses and effectively destroyed the spatial structure of the biofilms. The inhibition and removal effects became more significant with increasing sodium selenite concentrations.

Conclusion: We demonstrated that sodium selenite can inhibit and remove biofilms of mature mixed strains on voice prostheses, providing a novel basis for treating patients' voice prosthesis biofilms.

Keywords: inhibit; mature biofilms; mixed strains on voice prostheses; remove; sodium selenite; voice prostheses.

Grants and funding

This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences under Grant number XDA1601040201 and the Nature Science Foundation of Beijing under Grant number 7192171.