Bordetella pertussis causes whooping cough and is transmitted via respiratory droplets. Here, we present a protocol to challenge mice with Bordetella pertussis. We describe bacteria preparation and long-term storage, followed by manufacturing a challenge dose for use in a commercial exposure chamber with controlled nebulization of B. pertussis into aerosols. We then detail the aerosol challenge of mice through a more natural administration than intranasal instillation and post-challenge data collection. This protocol allows for better comparisons between preclinical pertussis studies.
Keywords: Health Sciences; Immunology; Microbiology; Model Organisms.
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