SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants

Linhao Ge; Buwei Cao; Rui Qiao; Hongguang Cui; Shaofang Li; Hongying Shan; Pan Gong; Mingzhen Zhang; Hao Li; Aiming Wang; Xueping Zhou; Fangfang Li

doi:10.1016/j.molp.2022.12.024

SUMOylation-modified Pelota-Hbs1 RNA surveillance complex restricts the infection of potyvirids in plants

Mol Plant. 2023 Mar 6;16(3):632-642. doi: 10.1016/j.molp.2022.12.024. Epub 2023 Jan 2.

Authors

Linhao Ge¹, Buwei Cao¹, Rui Qiao¹, Hongguang Cui², Shaofang Li¹, Hongying Shan¹, Pan Gong¹, Mingzhen Zhang¹, Hao Li¹, Aiming Wang³, Xueping Zhou⁴, Fangfang Li⁵

Affiliations

¹ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
² Key Laboratory of Green Prevention and Control of Tropical Plant Diseases and Pests, Ministry of Education and College of Plant Protection, Hainan University, Haikou, Hainan, China.
³ London Research and Development Centre, Agriculture and Agri-Food Canada, London, ON, Canada; Department of Biology, Western University, London, ON, Canada.
⁴ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China; State Key Laboratory of Rice Biology, Institute of Biotechnology, Zhejiang University, Hangzhou, Zhejiang, China. Electronic address: zzhou@zju.edu.cn.
⁵ State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China. Electronic address: lifangfang@caas.cn.

PMID: 36597359
DOI: 10.1016/j.molp.2022.12.024

Abstract

RNA quality control nonsense-mediated decay is involved in viral restriction in both plants and animals. However, it is not known whether two other RNA quality control pathways, nonstop decay and no-go decay, are capable of restricting viruses in plants. Here, we show that the evolutionarily conserved Pelota-Hbs1 complex negatively regulates infection of plant viruses in the family Potyviridae (termed potyvirids), the largest group of plant RNA viruses that accounts for more than half of the viral crop damage worldwide. Pelota enables the recognition of the functional G_1-2A_6-7 motif in the P3 cistron, which is conserved in almost all potyvirids. This allows Pelota to target the virus and act as a viral restriction factor. Furthermore, Pelota interacts with the SUMO E2-conjugating enzyme SCE1 and is SUMOylated in planta. Blocking Pelota SUMOylation disrupts the ability to recruit Hbs1 and inhibits viral RNA degradation. These findings reveal the functional importance of Pelota SUMOylation during the infection of potyvirids in plants.

Keywords: G(1-2)A(6-7) motif; Pelota-Hbs1; Potyviruses; SUMOylation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Nonsense Mediated mRNA Decay*
Plants
Potyvirus
RNA
Sumoylation*

Substances

RNA