Genes associated with desiccation stress in foodborne Staphylococcus aureus as revealed by transposon insertion mutagenesis

Abstract

Staphylococcus aureus (S. aureus) is an opportunistic foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. However, the molecular mechanisms involved in desiccation stress have received little attention in S. aureus. Here, some potential genes related to desiccation stress were determined in S. aureus by the transposon random mutagenesis approach. Eight mutants with different mutant sites who showed lower survival rates compared to wild-type (WT) strain RMSA24 under desiccation stress were successfully screened from a mutant library (n = 3,154). The eight mutation sites are identified as potential genes of U32 family peptidase, CHAP domain-containing protein, YdcF family protein, RNA polymerase sigma factor, EVE domain-containing protein, acetyltransferase, LPXTG-anchored DUF1542 repeat protein FmtB, and CvpA family protein, which haven't been reported as the desiccation-tolerant related genes. We found that the growth rates and biofilm formation abilities of these mutants were not significantly affected, indicating that their reduced survival rates under desiccation stress not dependent on reduced growth rates and biofilm formation abilities. Under desiccation stress, the expression levels of the three mutated genes were up-regulated and the four mutated genes were down-regulated in the WT strain, implying that these genes may play different roles in S. aureus to adapt to desiccation stress conditions. The study reveals valuable information for the control of S. aureus in low water activity foods and their production environments.

Keywords: Biofilm; Desiccation stress; Gene expression; Staphylococcus aureus; Transposon mutagenesis.