Nutritional properties and osteogenic activity of simulated digestion components and peptides from Larimichthys crocea

Zhe Xu; Shiying Han; Hui Chen; Lingyu Han; Xiufang Dong; Maolin Tu; Zhijian Tan; Ming Du; Tingting Li

doi:10.1016/j.foodres.2022.112238

Nutritional properties and osteogenic activity of simulated digestion components and peptides from Larimichthys crocea

Food Res Int. 2023 Jan:163:112238. doi: 10.1016/j.foodres.2022.112238. Epub 2022 Nov 28.

Authors

Zhe Xu¹, Shiying Han², Hui Chen³, Lingyu Han², Xiufang Dong⁴, Maolin Tu⁵, Zhijian Tan⁶, Ming Du⁷, Tingting Li⁸

Affiliations

¹ College of Life Sciences, Key Laboratory of Biotechnology and Bioresources Utilization, Dalian Minzu University, Ministry of Education, Dalian 116029, China; Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China.
² College of Life Sciences, Key Laboratory of Biotechnology and Bioresources Utilization, Dalian Minzu University, Ministry of Education, Dalian 116029, China.
³ Key Laboratory of Marine Fishery Resources Exploitment & Utilization of Zhejiang Province, Zhejiang University of Technology, Hangzhou 310014, China.
⁴ College of Marine Science and Biological Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
⁵ Key Laboratory of Animal Protein Food Deep Processing Technology of Zhejiang Province, College of Food and Pharmaceutical Sciences, Ningbo University, Ningbo 315832, China.
⁶ Institute of Bast Fiber Crops & Center of Southern Economic Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, China. Electronic address: tanzhijian@caas.cn.
⁷ School of Food Science and Technology, National Engineering Research Center of Seafood, Collaborative Innovation Center of Seafood Deep Processing, Dalian Polytechnic University, Dalian 116034, China. Electronic address: duming@dlpu.edu.cn.
⁸ College of Life Sciences, Key Laboratory of Biotechnology and Bioresources Utilization, Dalian Minzu University, Ministry of Education, Dalian 116029, China. Electronic address: jwltt@dlnu.edu.cn.

PMID: 36596160
DOI: 10.1016/j.foodres.2022.112238

Abstract

Fish provides a range of health benefits due to its nutritional and bioactive components. However, the bioactive peptides derived from Larimichthys crocea proteins were not fully investigated, especially the beneficial effects related to bone growth in vitro. In this study, the water extract protein was subjected to the simulated in vitro digestion process, and the osteogenic effect of enzymatic hydrolysate at different digestion stages was evaluated by the proliferation of osteoblast. The protein hydrolyzates of group pepsin treatment for 1 h and pepsin treatment for 2 h showed higher osteogenic activity in vitro. Two peptides including IERGDVVVQDSPSD from pepsin treatment for 1 h and RGDLGIEIPTEK from pepsin treatment for 2 h were identified, which revealed eminent effects in terms of promoting osteoblast proliferation and enhancing ALP activity. Moreover, the available nutrients in the proteins were determined by the molecular weight distribution and free amino acid composition. Those peptides also showed stronger interaction with RGD than integrins. Therefore, the peptides from Larimichthys crocea can be used as an effective ingredient for promoting bone growth in the future.

Keywords: Digestion; Function; Larimichthys crocea; Osteoblasts; Protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Digestion
Fishes / metabolism
Pepsin A* / metabolism
Peptides / chemistry
Perciformes*

Substances

Pepsin A
Peptides