Acidic hydrolyzed xylo-oligosaccharides bioactivity on the antioxidant and immune activities of macrophage

Xinlu Liu; Rou Cao; Yong Xu

doi:10.1016/j.foodres.2022.112152

Acidic hydrolyzed xylo-oligosaccharides bioactivity on the antioxidant and immune activities of macrophage

Food Res Int. 2023 Jan:163:112152. doi: 10.1016/j.foodres.2022.112152. Epub 2022 Nov 26.

Authors

Xinlu Liu¹, Rou Cao¹, Yong Xu²

Affiliations

¹ Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People's Republic of China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People's Republic of China.
² Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, People's Republic of China; Jiangsu Province Key Laboratory of Green Biomass-based Fuels and Chemicals, Nanjing 210037, People's Republic of China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, People's Republic of China. Electronic address: xuyong@njfu.edu.cn.

PMID: 36596103
DOI: 10.1016/j.foodres.2022.112152

Abstract

Xylo-oligosaccharides (XOS) prepared by the acetic acid hydrolysis of corncob were adulterated with many impurities including pigments, salts, and monosaccharides. Monosaccharides, acids, and most of the pigment were removed by a combination of decolorization, bipolar membrane electrodialysis and catalysis by Gluconobacter oxydans. These steps retain 90% of XOS in the acidolysis slurry. In this study, the effects of purified-XOS (PXOS) and crude XOS (CXOS) on the antioxidant and immune activities of macrophage were compared to verify the bioactivity of acidic hydrolyzed XOS, mainly focusing on the benefits of the purification process. PXOS was more effective in increasing superoxide dismutase activity and reducing malondialdehyde content, and thus had more potent antioxidant activity. In addition, PXOS could more efficiently promote the secretion of tumor necrosis factor-α, interleukin-6, nitric oxide, and interleukin-1β by macrophage. All these data, suggest that the purification process contributed to improve the immunomodulatory activity of XOS from acidolysis slurry.

Keywords: Acetic acid hydrolysis; Antioxidant activity; Immune activity; Macrophage; Purification; Xylo-oligosaccharides.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antioxidants* / pharmacology
Glucuronates*
Macrophages
Monosaccharides
Oligosaccharides / pharmacology

Substances

Antioxidants
Glucuronates
Oligosaccharides
Monosaccharides