Generation of iPSC-based human-mouse microglial brain chimeras to study senescence of human microglia

Mengmeng Jin; Ziyuan Ma; Peng Jiang

doi:10.1016/j.xpro.2022.101847

Generation of iPSC-based human-mouse microglial brain chimeras to study senescence of human microglia

STAR Protoc. 2022 Dec 16;3(4):101847. doi: 10.1016/j.xpro.2022.101847. Epub 2022 Nov 12.

Authors

Mengmeng Jin¹, Ziyuan Ma², Peng Jiang³

Affiliations

¹ Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA. Electronic address: mengmeng.jin@rutgers.edu.
² Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA.
³ Department of Cell Biology and Neuroscience, Rutgers University, Piscataway, NJ 08854, USA. Electronic address: peng.jiang@rutgers.edu.

Abstract

Here, we provide a step-by-step protocol for generating human induced pluripotent stem cell (hiPSC)-based microglial mouse brain chimeras. In addition, we detail steps for intracerebral injection of pathological tau and magnetic cell isolation of human microglia from chimeric mouse brains for single-cell RNA sequencing. Human microglia developed in chimeric mouse brains recapitulate the pathophysiology of microglia in human brain tissue, offering unprecedented opportunities to study human microglial senescence in vivo. For complete details on the use and execution of this protocol, please refer to (Jin et al., 2022b).

Keywords: Cell isolation; Model Organisms; Neuroscience; Single Cell; Stem Cells.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / physiology
Humans
Induced Pluripotent Stem Cells*
Mice
Microglia*

Abstract

Publication types

MeSH terms

Grants and funding