Characterization and epitope mapping of monoclonal antibodies against PEDV N protein

Yongxiang Zhao; Baochao Fan; Junbiao Xue; Rongli Guo; Jizhong Li; Jinzhu Zhou; Xu Song; Xuehan Zhang; Shengce Tao; Bin Li

doi:10.1016/j.virol.2022.12.011

Characterization and epitope mapping of monoclonal antibodies against PEDV N protein

Virology. 2023 Feb:579:29-37. doi: 10.1016/j.virol.2022.12.011. Epub 2022 Dec 29.

Authors

Yongxiang Zhao¹, Baochao Fan¹, Junbiao Xue², Rongli Guo¹, Jizhong Li¹, Jinzhu Zhou¹, Xu Song³, Xuehan Zhang¹, Shengce Tao⁴, Bin Li⁵

Affiliations

¹ Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China; Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China.
² Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China.
³ Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China; College of Veterinary Science, Hebei Agricultural University, Baoding, China.
⁴ Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Center for Systems Biomedicine, Shanghai Jiao Tong University, Shanghai, China. Electronic address: taosc@sjtu.edu.cn.
⁵ Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Nanjing, China; Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing, China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou, China. Electronic address: libinana@126.com.

PMID: 36592554
DOI: 10.1016/j.virol.2022.12.011

Abstract

Porcine epidemic diarrhea virus (PEDV) causes acute diarrhea, vomiting, dehydration and high mortality in neonatal piglets. The nucleocapsid (N) protein of PEDV is a highly conserved protein with strong immunogenicity and palys an important role in PEDV diagnosis. However, epitopes on the PEDV N protein have not yet been well characterized. Here, 32 monoclonal antibodies (mAbs) against the PEDV N protein were produced and identified. Six new epitopes were first identified by using a high-throughput epitope mapping method named AbMap. Sequence analysis revealed that among the six epitopes five epitopes were highly conserved among different PEDV strains. We also confirmed that the mAbs derived from the six epitopes of PEDV N protein, have no cross-reactivity with transmissible gastro enteritis virus or porcine delta coronavirus. These mAbs and their defined epitopes will help to understand the N protein structure and immunological characteristics, and to develop a rapid, accurate PEDV diagnosis method.

Keywords: Characterization; Epitope mapping; Monoclonal antibodies; Nucleocapsid; PEDV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antibodies, Monoclonal
Antibodies, Viral
Coronavirus Infections*
Epitope Mapping
Epitopes
Porcine epidemic diarrhea virus*
Swine
Swine Diseases*

Substances

Antibodies, Monoclonal
Antibodies, Viral
Epitopes