Cytosine Base Editing in Bacteria

Ye Liu; Yang Liu; Ping Zheng; Yu Wang; Meng Wang

doi:10.1007/978-1-0716-2879-9_17

Cytosine Base Editing in Bacteria

Methods Mol Biol. 2023:2606:219-231. doi: 10.1007/978-1-0716-2879-9_17.

Authors

Ye Liu^{1

2}, Yang Liu^{1

2}, Ping Zheng^{1

2}, Yu Wang^{3

4}, Meng Wang^{5

6}

Affiliations

¹ Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
² National Technology Innovation Center of Synthetic Biology, Tianjin, China.
³ Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China. wang_y@tib.cas.cn.
⁴ National Technology Innovation Center of Synthetic Biology, Tianjin, China. wang_y@tib.cas.cn.
⁵ Key Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China. wangmeng@tib.cas.cn.
⁶ National Technology Innovation Center of Synthetic Biology, Tianjin, China. wangmeng@tib.cas.cn.

PMID: 36592319
DOI: 10.1007/978-1-0716-2879-9_17

Abstract

Base editing is a new genome editing technology that enables DNA base mutations without requiring double-stranded DNA backbone cleavage or a donor template. It has been widely used for genome engineering of eukaryotic and prokaryotic microorganisms. In this chapter, we describe a routine protocol for cytosine base editing in two model bacteria Corynebacterium glutamicum and Bacillus subtilis. The protocol can be adapted to base editing in other bacteria with modifications.

Keywords: Bacillus subtilis; Base editing; CRISPR; Corynebacterium glutamicum; Cytidine deaminase; Genome editing.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria / genetics
CRISPR-Cas Systems*
Cytosine
DNA
Gene Editing* / methods
Mutation

Substances

Cytosine
DNA