The ratio of electron transport to assimilation (ETR/AN): underutilized but essential for assessing both equipment's proper performance and plant status

Abstract

ETR/A_N ratios should be in the range 7.5-10.5 for non-stressed C₃ plants. Ratios extremely out of this range can be reflecting both uncontrolled plant status and technical mistakes during measurements. We urge users to explicitly refer to this ratio in future studies as a proof for internal data quality control. For the last few decades, the use of infra-red gas-exchange analysers (IRGAs) coupled with chlorophyll fluorometers that allow for measurements of net CO₂ assimilation rate and estimates of electron transport rate over the same leaf area has been popularized. The evaluation of data from both instruments in an integrative manner can result in additional valuable information, such as the estimation of the light respiration, mesophyll conductance and the partitioning of the flux of electrons into carboxylation, oxygenation and alternative processes, among others. In this review, an additional and more 'straight' use of the combination of chlorophyll fluorescence and gas exchange-derived parameters is presented, namely using the direct ratio between two fully independently estimated parameters, electron transport rate (ETR)-determined by the fluorometer-and net CO₂ assimilation rate (A_N)-determined by the IRGA, i.e., the ETR/A_N ratio, as a tool for fast detection of incongruencies in the data and potential technical problems associated with them, while checking for the study plant's status. To illustrate this application, a compilation of 75 studies that reported both parameters for a total of 178 species under varying physiological status is presented. Values of ETR/A_N between 7.5 and 10.5 were most frequently found for non-stressed C₃ plants. C₄ species showed an average ETR/A_N ratio of 4.7. The observed ratios were larger for species with high leaf mass per area and for plants subjected to stressful factors like drought or nutritional deficit. Knowing the expected ETR/A_N ratio projects this ratio as a routinary and rapid check point for guaranteeing both the correct performance of equipment and the optimal/stress status of studied plants. All known errors associated with the under- or overestimation of ETR or A_N are summarized in a checklist that aims to be routinely used by any IRGA/fluorometer user to strength the validity of their data.

Keywords: Absorptance; Chlorophyll fluorescence; Electron transport rate; Gas exchange; Leaf mass per area (LMA); Photosynthesis.