The nanotechnological arena has revolutionized the diagnostic efficacies by investigating the protein corona. This displays provoking proficiencies in determining biomarkers and diagnostic fingerprints for early detection and advanced therapeutics. The green synthesized iron oxide nanoparticles were prepared via Withania coagulans and were well characterized using UV-visible spectroscopy, X-ray diffraction analysis, Fourier transform infrared spectroscopy, and nano-LC mass spectrophotometry. Iron oxides were rod-shaped with an average size of 17.32 nm and have crystalline properties. The as-synthesized nanotool mediated firm nano biointeraction with the proteins in treatment with nine different cancers. The resultant of the proteome series was filtered oddly that highlighted the variant proteins within the differentially expressed proteins on behalf of nano-bioinformatics. Further magnification focused on S13_N, RS15, RAB, and 14_3_3 domains and few abundant motifs that aid scanning biomarkers. The entire set of variant proteins contracting to common proteins elucidates the underlining mechanical proteins that are marginally assessed using the robotic nanotechnology. Additionally, the iron rods indirectly possess a prognostic effect in manipulating expression of proteins through a smarter route. Thereby, such biologically designed nanotools provide a dual approach for medical studies.
© 2022 The Authors. Published by American Chemical Society.