The role of microbial ecology in improving the performance of anaerobic digestion of sewage sludge

Christian Krohn; Leadin Khudur; Daniel Anthony Dias; Ben van den Akker; Catherine A Rees; Nicholas D Crosbie; Aravind Surapaneni; Denis M O'Carroll; Richard M Stuetz; Damien J Batstone; Andrew S Ball

doi:10.3389/fmicb.2022.1079136

The role of microbial ecology in improving the performance of anaerobic digestion of sewage sludge

Front Microbiol. 2022 Dec 14:13:1079136. doi: 10.3389/fmicb.2022.1079136. eCollection 2022.

Authors

Affiliations

¹ ARC Training Centre for the Transformation of Australia's Biosolids Resource, RMIT University, Bundoora, VIC, Australia.
² School of Health and Biomedical Sciences, Discipline of Laboratory Medicine, STEM College, RMIT University, Bundoora, VIC, Australia.
³ South Australian Water Corporation, Adelaide, SA, Australia.
⁴ Melbourne Water Corporation, Docklands, VIC, Australia.
⁵ Water Research Centre, School of Civil and Environmental Engineering, University of New South Wales, Sydney, NSW, Australia.
⁶ Australian Centre for Water and Environmental Biotechnology, Gehrmann Building, The University of Queensland, Brisbane, QLD, Australia.

Abstract

The use of next-generation diagnostic tools to optimise the anaerobic digestion of municipal sewage sludge has the potential to increase renewable natural gas recovery, improve the reuse of biosolid fertilisers and help operators expand circular economies globally. This review aims to provide perspectives on the role of microbial ecology in improving digester performance in wastewater treatment plants, highlighting that a systems biology approach is fundamental for monitoring mesophilic anaerobic sewage sludge in continuously stirred reactor tanks. We further highlight the potential applications arising from investigations into sludge ecology. The principal limitation for improvements in methane recoveries or in process stability of anaerobic digestion, especially after pre-treatment or during co-digestion, are ecological knowledge gaps related to the front-end metabolism (hydrolysis and fermentation). Operational problems such as stable biological foaming are a key problem, for which ecological markers are a suitable approach. However, no biomarkers exist yet to assist in monitoring and management of clade-specific foaming potentials along with other risks, such as pollutants and pathogens. Fundamental ecological principles apply to anaerobic digestion, which presents opportunities to predict and manipulate reactor functions. The path ahead for mapping ecological markers on process endpoints and risk factors of anaerobic digestion will involve numerical ecology, an expanding field that employs metrics derived from alpha, beta, phylogenetic, taxonomic, and functional diversity, as well as from phenotypes or life strategies derived from genetic potentials. In contrast to addressing operational issues (as noted above), which are effectively addressed by whole population or individual biomarkers, broad improvement and optimisation of function will require enhancement of hydrolysis and acidogenic processes. This will require a discovery-based approach, which will involve integrative research involving the proteome and metabolome. This will utilise, but overcome current limitations of DNA-centric approaches, and likely have broad application outside the specific field of anaerobic digestion.

Keywords: biomarker; biosolids; circular economy; foaming; meta-omics; microbial ecology; pollutant monitoring; renewable natural gas.

Publication types

Review