Detection of fluorescence-labeled DNA with in-plane organic optoelectronic devices

Igor Titov; Nils Rutschke; Fabio A Kraft; Markus Köpke; Eric Nebling; Martina Gerken

doi:10.1364/BOE.475358

Detection of fluorescence-labeled DNA with in-plane organic optoelectronic devices

Biomed Opt Express. 2022 Nov 8;13(12):6300-6316. doi: 10.1364/BOE.475358. eCollection 2022 Dec 1.

Authors

Igor Titov¹, Nils Rutschke², Fabio A Kraft¹, Markus Köpke¹, Eric Nebling³, Martina Gerken¹

Affiliations

¹ Integrated Systems and Photonics, Faculty of Engineering, Kiel University, Germany.
² altona Diagnostics GmbH, Hamburg, Germany.
³ Battery Systems for Special Applications, Fraunhofer Institute for Silicon Technology, Germany.

Abstract

We present a system efficiency analysis of a monolithic integrated organic optoelectronic unit for the detection of fluorescence labeled single-stranded DNA (ssDNA) for veterinary disease testing. The side-by-side integration of an organic light emitting diode (OLED) and an organic photodetector (OPD) with 0.5 mm by 0.5 mm device sizes has the potential to enable compact and low-cost fluorescence point-of-care (POC) devices for decentral multiplex biomedical testing. Here, we used two 6-FAM and BHQ1 labeled complementary ssDNA strands to form the Förster resonance transfer (FRET) upon the hybridization of the DNA. In this work we successfully show ssDNA hybridization sensing with samples diluted in TE buffer and investigate the detection of covalently bound 6-FAM-ssDNA on a glass surface for multiplex biomarker measurements.