Chlorophyll a fluorescence (ChF) signal analysis has become a widely used and rapid, non-invasive technique to study the photosynthetic process under stress conditions. It monitors plant responses to various environmental factors affecting plants under experimental and field conditions. Thus, it enables extensive research in ecology and benefits forestry, agriculture, horticulture, and arboriculture. Woody plants, especially trees, as organisms with a considerable life span, have a different life strategy than herbaceous plants and show more complex responses to stress. The range of changes in photosynthetic efficiency of trees depends on their age, ontogeny, species-specific characteristics, and acclimation ability. This review compiles the results of the most commonly used ChF techniques at the foliar scale. We describe the results of experimental studies to identify stress factors that affect photosynthetic efficiency and analyse the experience of assessing tree vigour in natural and human-modified environments. We discuss both the circumstances under which ChF can be successfully used to assess woody plant health and the ChF parameters that can be useful in field research. Finally, we summarise the advantages and limitations of the ChF method in research on trees, shrubs, and woody vines.
Keywords: JIP-test; PAM fluorescence; forests; shrubs; trees; urban trees.
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