Changes of Pepsin Concentration in Saliva Sample According to Storage Period

Hee Jin Kang; Joo Kyung Noh; Min Kyeong Lee; Seon Rang Woo; Jung Min Park; Young Chan Lee; Seong-Gyu Ko; Young-Gyu Eun

doi:10.1016/j.jvoice.2022.12.010

Changes of Pepsin Concentration in Saliva Sample According to Storage Period

J Voice. 2022 Dec 30:S0892-1997(22)00411-8. doi: 10.1016/j.jvoice.2022.12.010. Online ahead of print.

Authors

Hee Jin Kang¹, Joo Kyung Noh², Min Kyeong Lee², Seon Rang Woo³, Jung Min Park¹, Young Chan Lee¹, Seong-Gyu Ko⁴, Young-Gyu Eun⁵

Affiliations

¹ Department of Otolaryngology Head & Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea.
² Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, Republic of Korea.
³ Department of Otolaryngology Head & Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea; Central Laboratory, Medical Science Research Institute, Kyung Hee University Medical Center, Seoul, Republic of Korea.
⁴ Department of Preventive Medicine, College of Korean Medicine, Kyung Hee University, Seoul, Republic of Korea.
⁵ Department of Otolaryngology Head & Neck Surgery, Kyung Hee University School of Medicine, Kyung Hee University Medical Center, Seoul, Republic of Korea; Department of Biomedical Science and Technology, Graduate School, Kyung Hee University, Seoul, Republic of Korea. Electronic address: ygeun@khu.ac.kr.

PMID: 36588013
DOI: 10.1016/j.jvoice.2022.12.010

Abstract

Objectives: To determine whether the concentration of pepsin in the saliva sample changes according to the storage period of the sample.

Methods: Forty eight patients with suspected laryngopharyngeal reflux were included in this study. Saliva samples were collected from each patient and each sample divided into six and stored for different period of time. Pepsin concentration was measured using enzyme-linked immunosorbent assay. A comparison was made between the pepsin concentration measured immediately and the concentration measured after storage for 1 week, 2 weeks, 1 month, 3 months, and 6 months.

Results: No significant difference in pepsin concentrations were detected between the sample analyzed immediately and those analyzed at 1 week, 2 weeks, 1 month, and 3 months after saliva collection. A significant difference in pepsin concentration was observed in the sample analyzed immediately and the sample analyzed 6 months after saliva collection.

Conclusions: Pepsin concentration in saliva samples did not demonstrate a significant difference between the concentration measured immediately and the concentration measured 3 months after saliva collection, although a significant difference was observed in the concentration measured 6 months after collection.

Keywords: Diagnosis; Laryngopharyngeal reflux; Pepsin; Saliva; Storage.