Cytotoxic steroidal glycosides from the rhizomes of Paris polyphylla var. yunnanensis

Yang Liu; Mei-You Liu; Lin-Lin Bi; Yun-Yuan Tian; Peng-Cheng Qiu; Xiao-Ying Qian; Min-Chang Wang; Hai-Feng Tang; Yun-Yang Lu; Bang-Le Zhang

doi:10.1016/j.phytochem.2022.113577

Cytotoxic steroidal glycosides from the rhizomes of Paris polyphylla var. yunnanensis

Phytochemistry. 2023 Mar:207:113577. doi: 10.1016/j.phytochem.2022.113577. Epub 2022 Dec 29.

Authors

Yang Liu¹, Mei-You Liu², Lin-Lin Bi³, Yun-Yuan Tian³, Peng-Cheng Qiu³, Xiao-Ying Qian⁴, Min-Chang Wang⁵, Hai-Feng Tang⁶, Yun-Yang Lu⁷, Bang-Le Zhang⁸

Affiliations

¹ Department of Pharmaceutics, School of Pharmacy, Air Force Medical University, Xi'an, China.
² Department of Pharmacy, Xijing Hospital, Fourth Military Medical University, Xi'an, China.
³ Department of Chinese Materia Medica and Natural Medicines, School of Pharmacy, Air Force Medical University, Xi'an, China.
⁴ Center for Chemical Biology, Institute of Interdisciplinary Integrative Medicine Research, Shanghai University of Traditional Chinese Medicine, Shanghai, China.
⁵ Xi'an Modern Chemistry Research Institute, Xi'an, China.
⁶ Department of Chinese Materia Medica and Natural Medicines, School of Pharmacy, Air Force Medical University, Xi'an, China. Electronic address: tanghaifeng71@163.com.
⁷ Department of Chinese Materia Medica and Natural Medicines, School of Pharmacy, Air Force Medical University, Xi'an, China. Electronic address: luyunyanggq@163.com.
⁸ Department of Pharmaceutics, School of Pharmacy, Air Force Medical University, Xi'an, China. Electronic address: blezhang@fmmu.edu.cn.

PMID: 36587887
DOI: 10.1016/j.phytochem.2022.113577

Abstract

Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz. (Melanthiaceae), an important specie of the genus Paris, has long been in a traditional Chinese medicine (TCM) for a long time. This study aimed to isolate and identify the structures of bioactive saponins from the rhizomes of P. polyphylla var. yunnanensis and evaluate their cytotoxicity against BxPC-3, HepG2, U373 and SGC-7901 carcinoma cell lines. Seven previously undescribed and seven known saponins were identified, and Paris saponins VII (PSVII) showed significant cytotoxicity against the BxPC-3 cell line with IC₅₀ values of 3.59 μM. Furthermore, flow cytometry, transmission electron microscopy and western-bolt analysis revealed that PSVII inhibited the proliferation of BxPC-3 cells and might be involved in inducing apoptosis and pyroptosis by activating caspase-3, -7 and caspase-1, respectively.

Keywords: Apoptosis; Cytotoxicity; Furostanol saponins; Melanthiaceae; Paris polyphylla var. yunnanensis (Franch.) Hand.-Mazz.; Pyroptosis; Saponins; Spirostane saponin.

MeSH terms

Antineoplastic Agents*
Liliaceae* / chemistry
Melanthiaceae* / chemistry
Rhizome / chemistry
Saponins* / pharmacology

Substances

Saponins
Antineoplastic Agents