Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber

Jason Sedlak; Alisa Clyne

doi:10.1007/978-1-0716-2851-5_5

Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber

Methods Mol Biol. 2023:2600:81-90. doi: 10.1007/978-1-0716-2851-5_5.

Authors

Jason Sedlak¹, Alisa Clyne²

Affiliations

¹ Drexel University, Philadelphia, PA, USA.
² University of Maryland, College Park, MD, USA. aclyne@umd.edu.

PMID: 36587091
DOI: 10.1007/978-1-0716-2851-5_5

Abstract

Endothelial cell response to shear stress from the flowing blood plays an important role in diseases such as atherosclerosis. It is therefore essential to study how different flow regimes, including both atheroprotective laminar flow and atheropromoting disturbed flow, impact endothelial cell function. Shear stress effects can be studied in vitro using a variety of techniques, each with their own advantages and disadvantages. In this chapter, we describe how to use a parallel plate flow chamber to study the impact of both laminar and disturbed flow on endothelial cell monolayers.

Keywords: Atherosclerosis; Endothelial cells; Flow chamber; Shear stress.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Atherosclerosis*
Cells, Cultured
Endothelial Cells* / physiology
Endothelium, Vascular / physiology
Humans
Stress, Mechanical

Abstract

Publication types

MeSH terms

Grants and funding