Glycosphingolipids (GSLs) are glycolipids with ceramide and carbohydrate head groups that play an important role in numerous biological processes. Previously, we performed GSL-glycan analysis of various cell lines and virus-infected cells using a glycoblotting approach. Recently, we developed several methods for sialic acid linkage-specific chemical modification to distinguish sialylated glycan isomers by mass spectrometry. In this chapter, we describe a method for analyzing GSL-glycans in human serum/plasma using glycoblotting combined with aminolysis-SALSA (sialic acid linkage-specific alkylamidation) and lactone-driven ester-to-amide derivatization (LEAD)-SALSA for comprehensive and detailed structural glycomics.
Keywords: Glycoblotting; Glycosphingolipid-glycan; Human plasma; Human serum; Lactone-driven ester-to-amide derivatization; Mass spectrometry; Sialic acid linkage-specific alkylamidation.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.