Massilia phyllostachyos sp. nov., Isolated from the Roots of Moso Bamboo in China

Abstract

A Gram-negative, strictly aerobic, motile, and rod-shaped bacterial strain G4R7^T was isolated from the roots of moso bamboo (Phyllostachys edulis) in Zhejiang, Hangzhou Province, China. After comparing 16S rRNA gene sequences, strain G4R7^T exhibited the highest similarities with Massilia neuiana PTW21^T (98.3%), followed by M. agri K-3-1^T (98.3%), M. consociate CCUG 58010^T (97.7%), M. niastensis 5516S-1^T (97.7%) and M. yuzhufengensis ZD1-4^T (97.6%). The phylogenetic analysis revealed that strain G4R7^T belonged to the genus Massilia. The draft genome of strain G4R7^T was 5.81 Mb, and the G+C content was 64.4%. The average nucleotide identity values between G4R7^T and another related member of the genus Massilia ranged from 76.6 to 87.2%, and the digital DNA-DNA hybridization ranged from 20.7 to 27.9%. Strain G4R7^T grew at 15-37 °C (optimum 25-30 °C) and pH 6.0-9.0 (optimum pH 7.0) in the presence of 0-3% (w/v) NaCl (optimum 0%). The respiratory quinone was Q-8, and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, and aminophospholipid. The major cellular fatty acids were C_10:0 3OH, C_12:0, C_12:0 2OH, and C_16:0, summed feature 3 (C_16:1 ω6c and/or C_16:1 ω7c). As per the data from chemotaxonomic, phylogenetic, and phenotypic evidence, strain G4R7^T represents a new species of genus Massilia, for which the name Massilia phyllostachyos sp. nov. is proposed. The type strain is G4R7^T (=ACCC 61911^T=GDMCC 1.2961^T=JCM 35225^T).