Biofilm elimination from infected root canals using four different single files

Sarah A Hamed; Sarah Shabayek; Hayam Y Hassan

doi:10.1186/s12903-022-02690-5

Biofilm elimination from infected root canals using four different single files

BMC Oral Health. 2022 Dec 31;22(1):660. doi: 10.1186/s12903-022-02690-5.

Authors

Sarah A Hamed¹, Sarah Shabayek², Hayam Y Hassan³

Affiliations

¹ Endodontic Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt.
² Microbiology and Immunology Department, Faculty of Pharmacy, Suez Canal University, Ismailia, Egypt.
³ Endodontic Department, Faculty of Dentistry, Suez Canal University, Ismailia, Egypt. hayam_youssef@dent.suez.edu.eg.

Abstract

Introduction: Enterococcus faecalis (E. faecalis) is the most commonly isolated bacterium from infected root canals. It is found in the form of a biofilm, which makes it more resistant to antimicrobials, and requires optimal chemomechanical strategies to maximize root canal disinfection.

Aim: To evaluate the efficacy of 4 different endodontic file systems against E. faecalis biofilm growth in root canals using colony-forming units per milliliter (CFU/mL) and scanning electron microscope (SEM).

Methods: Eighty-five extracted human mandibular premolars with straight root canals and apical diameters not larger than the #15 K-file were randomly selected. After performing a pilot study (n = 15) to determine the ideal incubation period for E. faecalis biofilm development, sixty-five root canals were infected with E. faecalis, incubated for 3 weeks, and then mechanically prepared using one of four single files (XP-endo Shaper, Hyflex EDM, One Curve, and Fanta. AFTM F One) (n = 15). Five infected root canals were excluded for the positive control. Five non-contaminated root canals were included for the negative control. Samples were collected using sterile paper points pre- and post-instrumentation to determine the bacterial load (CFU/mL). Root canals from each group were topographically evaluated at the coronal, middle, and apical segments using scanning electron microscope (SEM). Bacterial reduction data were estimated and statistically analyzed by Kruskal-Wallis and Mann-Whitney U tests (post hoc test) (P ≤ .05).

Results: XP-endo Shaper, Hyflex DEM, and One Curve significantly could eradicate E. faecalis biofilms in infected root canals with no significant difference among them compared to Fanta. AF™ F One.

Conclusion: None of the systems were capable of completely eliminating biofilms. XP-endo Shaper, Hyflex EDM, and One Curve mechanically eliminated E. faecalis biofilms compared to Fanta. AF™ F One from infected root canals.

Keywords: Enterococcus faecalis biofilm; Hyflex DEM; One curve; XP-endo shaper.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Biofilms
Dental Pulp Cavity* / microbiology
Enterococcus faecalis
Humans
Pilot Projects
Root Canal Irrigants
Root Canal Preparation*
Root Canal Therapy

Substances

Root Canal Irrigants