Immunochromatographic assay for the analysis of methomyl in cabbage and tomato

Jie Liu; Lingling Guo; Aihong Wu; Shanshan Song; Liqiang Liu; Chuanlai Xu; Hua Kuang; Liguang Xu

doi:10.1016/j.foodchem.2022.135273

Immunochromatographic assay for the analysis of methomyl in cabbage and tomato

Food Chem. 2023 May 30:409:135273. doi: 10.1016/j.foodchem.2022.135273. Epub 2022 Dec 22.

Authors

Jie Liu¹, Lingling Guo¹, Aihong Wu¹, Shanshan Song¹, Liqiang Liu¹, Chuanlai Xu², Hua Kuang¹, Liguang Xu³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. Electronic address: xcl@jiangnan.edu.cn.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China; International Joint Research Laboratory for Biointerface and Biodetection, and School of Food Science and Technology, Jiangnan University, Wuxi, People's Republic of China. Electronic address: xlg@jiangnan.edu.cn.

PMID: 36584534
DOI: 10.1016/j.foodchem.2022.135273

Abstract

In this study, a hapten of methomyl was designed and used to produce monoclonal antibodies (mAbs) against methomyl. Based on these mAbs, we developed an enzyme-linked immunosorbent assay (ELISA) and immunochromatographic assay (ICA) strip for the determination of methomyl residues. Results from the ELISA showed that mAb 1D10 exhibited higher affinity with an affinity constant of 2.76 × 10¹⁰ L/mol and higher sensitivity with a limit of detection (LOD) was 8.12 ng/mL. After optimizing the ICA, a visible limit of detection (vLOD) was found to be 100 ng/g and the cut-off value was 500 ng/g for methomyl in cabbage and tomato. The calculated LODs were 3.2 ng/g and 5.4 ng/g in cabbage and tomato, respectively. Moreover, results from the ICA were consistent with those of the ELISA in our recovery assay using spiked samples. Hence, the ICA method has a bright future and great prospects for the detection of methomyl in food samples.

Keywords: Cabbage; Immunochromatographic assay; Methomyl; Monoclonal antibody; Tomato.

MeSH terms

Antibodies, Monoclonal
Brassica*
Chromatography, Affinity / methods
Enzyme-Linked Immunosorbent Assay / methods
Immunoassay / methods
Limit of Detection
Methomyl
Solanum lycopersicum*

Substances

Methomyl
Antibodies, Monoclonal