Performance of wild, tailed, humidity-robust phage on a surface-scanning magnetoelastic biosensor for Salmonella Typhimurium detection

Hwa-Eun Lee; Yu-Bin Jeon; Bryan A Chin; Sang Hyuk Lee; Hye Jin Lee; Mi-Kyung Park

doi:10.1016/j.foodchem.2022.135239

Performance of wild, tailed, humidity-robust phage on a surface-scanning magnetoelastic biosensor for Salmonella Typhimurium detection

Food Chem. 2023 May 30:409:135239. doi: 10.1016/j.foodchem.2022.135239. Epub 2022 Dec 17.

Authors

Hwa-Eun Lee¹, Yu-Bin Jeon¹, Bryan A Chin², Sang Hyuk Lee³, Hye Jin Lee³, Mi-Kyung Park⁴

Affiliations

¹ School of Food Science and Biotechnology, and Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea.
² Department of Materials Engineering, and Material Research and Education Center, Auburn University, Auburn, AL 36849, USA.
³ Department of Chemistry and Green-Nano Materials Research Center, Kyungpook National University, Daegu 41566, Republic of Korea.
⁴ School of Food Science and Biotechnology, and Food and Bio-Industry Research Institute, Kyungpook National University, Daegu 41566, Republic of Korea. Electronic address: parkmik@knu.ac.kr.

PMID: 36584528
DOI: 10.1016/j.foodchem.2022.135239

Abstract

A wild, tailed phage (TST) was compared with a genetically modified, filamentous phage (FST) for S. Typhimurium (ST) detection. When both phages were introduced into oppositely charged MUA and MUAM sensors, the RU values of TST showed an obvious increase on the MUAM sensor. The sensitivity of TST [54.78 ΔRU/(log PFU/mL)] was greater than that of FST [48.05 ΔRU/(log PFU/mL)]. The binding affinity (K_D = 1.75 × 10^-13 M) of TST on MUAM sensor was greater than that of FST. Both phages were specific to only ST, and TST exhibited a persistent binding capability at 50 % RH. When each phage-immobilized sensor was employed on chili pepper, the sensitivity [880.80 Hz/(log CFU/mL)] and detection limit (1.31 ± 0.27 log CFU/mL) of TST were significantly greater than those of FST. The orientation of TST on sensor promoted the uniform capture of bacteria and enhanced the reliable performance of a surface-scanning magnetoelastic biosensor.

Keywords: Binding interaction; Filamentous phage; Surface-scanning coil; Tailed phage; Δf(SEM); Δf(measured).

MeSH terms

Bacteriophages* / genetics
Biosensing Techniques*
Capsicum*
Humidity
Salmonella typhimurium / genetics