Data on genome resequencing of the rice cultivar MR219 and its abiotic stress tolerant mutant lines, NMR152 and NMR151

Faiz Ahmad; Nor'Aishah Hasan; Siti Norvahida Hisham; Siti Nurdiyana Yusof; Affrida Abu Hassan; Sobri Hussein; Abdul Rahim Harun; Noraziyah Abd Aziz Shamsudin

doi:10.1016/j.dib.2022.108815

Data on genome resequencing of the rice cultivar MR219 and its abiotic stress tolerant mutant lines, NMR152 and NMR151

Data Brief. 2022 Dec 13:46:108815. doi: 10.1016/j.dib.2022.108815. eCollection 2023 Feb.

Authors

Faiz Ahmad^{1

2}, Nor'Aishah Hasan³, Siti Norvahida Hisham¹, Siti Nurdiyana Yusof^{1

2}, Affrida Abu Hassan², Sobri Hussein², Abdul Rahim Harun², Noraziyah Abd Aziz Shamsudin^{1

4}

Affiliations

¹ Department of Biological Sciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.
² Agrotechnology and Biosciences Division, Malaysian Nuclear Agency, Bangi, 43000, Kajang, Selangor, Malaysia.
³ Faculty of Applied Sciences, Universiti Teknologi MARA, Cawangan Negeri Sembilan Kampus Kuala Pilah, 72000 Kuala Pilah, Negeri Sembilan, Malaysia.
⁴ Seed Bank Unit, Natural History Museum, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Selangor, Malaysia.

Abstract

The genomics and genetic information of Malaysian rice (Oryza sativa L.) and their mutant lines are very limited. Therefore, the genome resequencing of two drought and submergence tolerant mutant rice lines, NMR152 and NMR151 and their parental line, MR219 was performed using Illumina Hi Seq 4000 platform with 20x sequencing coverage to facilitate the identification of causal mutations and the discovery of candidate variants of single nucleotide polymorphisms (SNPs) from their genome. The identification of SNPs would be useful in developing functional SNP markers related to drought and submergences resistance traits. Genome sequence data (FASTQ format) of the mutants and their parental line have been deposited into the National Center for Biotechnology Information (NCBI) database under sequence read archive (SRA) data with accessions SRR20824202 (NMR152), SRR20995190 (NMR151) and SRR21043964 (MR219), respectively.

Keywords: Mutants; Next generation sequencing; Oryza sativa L.; Parental line; Raw sequence read.