Melanoidins, the brown late-stage Maillard reaction products, are responsible for color development and refractoriness in thermal hydrolyzed sludge (THS), causing negative effects on wastewater treatment. This study aimed to develop a methodology for the identification, isolation and preliminary characterization of the THS melanoidins. After thermal hydrolysis, the formation of melanoidins were confirmed by physicochemical indicators and excitation-emission matrix fluorescence analysis. The macroporous resin adsorption method was adopted to successfully extract melanoidins from THS with high recovery and selectivity. The main chemical components of the extracted melanoidins were carbohydrate (23.1 %), protein (43.8 %) and phenol (13.7 %), and the C/N was 4.5. In addition, furans, alcohols and sulfur-containing volatile substances were detected by pyrolysis-gas chromatography-mass spectrometry. Fourier transform infrared spectroscopy determined that functional groups such as CO, CN, NH, C-O-C, amide I and phenyl were present in the structure of THS melanoidins, and nuclear magnetic resonance spectroscopy indicated the formation of heterocyclic macromolecular structures. Their formation pathways were speculated to involve the cross-linkage of low-molecular-weight components (e.g. proteins, Amadori and Schiff base compounds) and the polymerization of heterocyclic units (e.g. furans, pyroles and pyrazines). The above results clarify the fundamental characteristics of the melanoidins formed during sludge thermal hydrolysis and will help improve subsequent research on melanoidins control.
Keywords: Chemical composition; Macroporous resin adsorption; Maillard reaction; Structure; Thermal hydrolysis pretreatment.
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