Enhanced production of high-value polyunsaturated fatty acids (PUFAs) from potential thraustochytrid Aurantiochytrium sp

Ajeet Singh Chauhan; Anil Kumar Patel; Chiu-Wen Chen; Jo-Shu Chang; Philippe Michaud; Cheng-Di Dong; Reeta Rani Singhania

doi:10.1016/j.biortech.2022.128536

Enhanced production of high-value polyunsaturated fatty acids (PUFAs) from potential thraustochytrid Aurantiochytrium sp

Bioresour Technol. 2023 Feb:370:128536. doi: 10.1016/j.biortech.2022.128536. Epub 2022 Dec 26.

Authors

Ajeet Singh Chauhan¹, Anil Kumar Patel², Chiu-Wen Chen³, Jo-Shu Chang⁴, Philippe Michaud⁵, Cheng-Di Dong⁶, Reeta Rani Singhania⁷

Affiliations

¹ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan.
² Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Centre for Energy and Environmental Sustainability, Lucknow-226 029, Uttar Pradesh, India.
³ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan.
⁴ Department of Chemical and Materials Engineering, Tunghai University, Taiwan; Research Center for Smart Sustainable Circular Economy, Tunghai University, Taiwan; Department of Chemical Engineering, National Cheng Kung University, Taiwan.
⁵ Université Clermont Auvergne, CNRS, SIGMA Clermont, Institute Pascal, 63000 Clermont-Ferrand, France.
⁶ Institute of Aquatic Science and Technology, College of Hydrosphere, National Kaohsiung University of Science and Technology, Kaohsiung City 81157, Taiwan; Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan. Electronic address: cddong@nkust.edu.tw.
⁷ Centre for Energy and Environmental Sustainability, Lucknow-226 029, Uttar Pradesh, India; Department of Marine Environmental Engineering, National Kaohsiung University of Science and Technology, Kaohsiung City, Taiwan.

PMID: 36581232
DOI: 10.1016/j.biortech.2022.128536

Abstract

Due to growing health concerns, the urban population is utterly inclined towards a healthy lifestyle and incorporated nutritional food supplements to lower common health risks. The ω-3 and ω-6 PUFAs consumption is increasing, hence alternative commercial production is essentially developed. The microbial source is an emerging platform to overcome the global demand for omega PUFAs. Marine oleaginous protist Aurantiochytrium sp. found a potential source to produce substantial DHA and SFA. The objective of the present research was to enhance the PUFA yield by optimizing maximum tolerable glucose concentration with a suitable nitrogen ratio (10:1). The maximum lipid and DHA yield and content were determined 4.30, 1.34 g/L, and 62.4, 33.49 % of total biomass and lipid at 30 g/L glucose respectively, which is one of among highest reported, however relative PUFA was maximum 46.97 % (DHA) in total lipid at 10 g/L glucose. Remaining 42-53.6 % SFA could be used for biodiesel.

Keywords: DHA; Lipid; Omega fatty acids; Poly unsaturated fatty acids; Thraustochytrid.

MeSH terms

Dietary Supplements
Docosahexaenoic Acids
Fatty Acids
Fatty Acids, Omega-3*
Fatty Acids, Unsaturated
Glucose
Stramenopiles*

Substances

Docosahexaenoic Acids
Fatty Acids, Omega-3
Fatty Acids, Unsaturated
Glucose
Fatty Acids