Shifting biomass allocation and light limitation co-regulate the temporal stability of an alpine meadow under eutrophication

Abstract

Eutrophication generally promotes but destabilizes grassland productivity. Under eutrophication, plants tend to decrease biomass allocation to roots but increase aboveground allocation and light limitation, likely affecting community stability. However, it remains unclear to understand how shifting plant biomass allocation and light limitation regulate grassland stability in response to eutrophication. Here, using a 5-yr multiple nutrient addition experiment in an alpine meadow, we explored the role of changes in plant biomass allocation and light limitation on its community stability under eutrophication as well as traditionally established mechanisms (i.e., plant Shannon diversity, species asynchrony and grass subcommunity stability). Our results showed that nitrogen (N) addition, rather than phosphorus (P) or potassium (K) addition, significantly reduced the temporal stability of the alpine meadow. In accordance with previous studies, we found that N addition decreased plant Shannon diversity, species asynchrony and grass subcommunity stability, further destabilizing meadow community productivity. In addition, we also found the decrease in biomass allocation to belowground by N addition, further weakening its community stability. Moreover, this shifts in plant biomass allocation from below- to aboveground, intensifying plant light limitation. Further, the light limitation reduced plant species asynchrony, which finally weakened its community stability. Overall, in addition to traditionally established mechanisms, this study highlights the role of plant biomass allocation shifting from belowground to aboveground in determining grassland community stability. These "unseen" mechanisms might improve our understanding of grassland stability in the context of ongoing eutrophication.

Keywords: Light limitation; Nutrient enrichment; Plant biomass allocation; Plant community stability; Plant species asynchrony; Plant species diversity.