Volatile organic compounds from Lysinibacillus macroides regulating the seedling growth of Arabidopsis thaliana

Dongying Zhao; Junhui Jiao; Binghai Du; Kai Liu; Chengqiang Wang; Yanqin Ding

doi:10.1007/s12298-022-01268-3

Volatile organic compounds from Lysinibacillus macroides regulating the seedling growth of Arabidopsis thaliana

Physiol Mol Biol Plants. 2022 Dec;28(11-12):1997-2009. doi: 10.1007/s12298-022-01268-3. Epub 2022 Dec 20.

Authors

Dongying Zhao¹, Junhui Jiao¹, Binghai Du^{1

2

3}, Kai Liu^{1

2}, Chengqiang Wang^{1

2}, Yanqin Ding^{1

2

3}

Affiliations

¹ College of Life Sciences, Shandong Agricultural University, Tai'an, 271018 China.
² Shandong Key Laboratory of Agricultural Microbiology, Tai'an, 271018 China.
³ Shandong Engineering Research Center of Plant-Microbia Restoration for Saline-Alkali Land, Tai'an, 271018 China.

Abstract

Volatile organic compounds (VOCs) have the characteristics of long distance propagation, low concentration, perception, and indirect contact between organisms. In this experiment, Lysinibacillus macroides Xi9 was isolated from cassava residue, and the VOCs produced by this strain were analyzed by the SPME-GC-MS method, mainly including alcohols, esters, and alkanes. By inoculation of L. macroides Xi9, VOCs can promote the growth and change the root-system architecture of Arabidopsis seedlings. The results showed that the number of lateral roots, root density, and fresh weight of Arabidopsis seedlings were significantly higher (p ≤ 0.01), and the number of roots hair was also increased after exposure to strain Xi9. Compared with the control group, the transcriptome analysis of Arabidopsis seedlings treated with strain Xi9 for 5 days revealed a total of 508 genes differentially expressed (p < 0.05). After Gene Ontology enrichment analysis, it was found that genes encoding nitrate transport and assimilation, and the lateral root-related gene ANR1 were up-regulated. The content of NO₃ ^- and amino acid in Arabidopsis seedlings were significantly higher from control group (p ≤ 0.01). Plant cell wall-related EXPA family genes and pectin lyase gene were up-regulated, resulting cell elongation of leaf. SAUR41 and up-regulation of its subfamily members, as well as the down-regulation of auxin efflux carrier protein PILS5 and auxin response factor 20 (ARF20) led to the accumulation of auxin. These results indicated that VOCs of strain Xi9 promote Arabidopsis seedlings growth and development by promoting nitrogen uptake, regulating auxin synthesis, and improving cell wall modification.

Supplementary information: The online version contains supplementary material available at 10.1007/s12298-022-01268-3.

Keywords: Auxin; Cell wall modification; Lysinibacillus macroides; Root-system architecture; Transcriptome; VOCs.

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