Heat and drought induced transcriptomic changes in barley varieties with contrasting stress response phenotypes

Ramamurthy Mahalingam; Naveen Duhan; Rakesh Kaundal; Andrei Smertenko; Taras Nazarov; Phil Bregitzer

doi:10.3389/fpls.2022.1066421

Heat and drought induced transcriptomic changes in barley varieties with contrasting stress response phenotypes

Front Plant Sci. 2022 Dec 8:13:1066421. doi: 10.3389/fpls.2022.1066421. eCollection 2022.

Authors

Ramamurthy Mahalingam¹, Naveen Duhan², Rakesh Kaundal², Andrei Smertenko³, Taras Nazarov³, Phil Bregitzer⁴

Affiliations

¹ Cereal Crops Research Unit, USDA-ARS, Madison, WI, United States.
² Department of Plant, Soils and Climate, Utah State University, Logan, UT, United States.
³ Institute of Biological Chemistry, Washington State University, Pullman, WA, United States.
⁴ National Small Grains Germplasm Research Facility, USDA-ARS, Aberdeen, ID, United States.

Abstract

Drought and heat stress substantially impact plant growth and productivity. When subjected to drought or heat stress, plants exhibit reduction in growth resulting in yield losses. The occurrence of these two stresses together intensifies their negative effects. Unraveling the molecular changes in response to combined abiotic stress is essential to breed climate-resilient crops. In this study, transcriptome profiles were compared between stress-tolerant (Otis), and stress-sensitive (Golden Promise) barley genotypes subjected to drought, heat, and combined heat and drought stress for five days during heading stage. The major differences that emerged from the transcriptome analysis were the overall number of differentially expressed genes was relatively higher in Golden Promise (GP) compared to Otis. The differential expression of more than 900 transcription factors in GP and Otis may aid this transcriptional reprogramming in response to abiotic stress. Secondly, combined heat and water deficit stress results in a unique and massive transcriptomic response that cannot be predicted from individual stress responses. Enrichment analyses of gene ontology terms revealed unique and stress type-specific adjustments of gene expression. Weighted Gene Co-expression Network Analysis identified genes associated with RNA metabolism and Hsp70 chaperone components as hub genes that can be useful for engineering tolerance to multiple abiotic stresses. Comparison of the transcriptomes of unstressed Otis and GP plants identified several genes associated with biosynthesis of antioxidants and osmolytes were higher in the former that maybe providing innate tolerance capabilities to effectively combat hostile conditions. Lines with different repertoire of innate tolerance mechanisms can be effectively leveraged in breeding programs for developing climate-resilient barley varieties with superior end-use traits.

Keywords: Barley; RNA-seq; combined stress; differential gene expression; drought; gene networks; gene ontologies; heat.