Fabrication of Antibacterial TiO2 Nanostructured Surfaces Using the Hydrothermal Method

Niharika Rawat; Metka Benčina; Ekaterina Gongadze; Ita Junkar; Aleš Iglič

doi:10.1021/acsomega.2c06175

Fabrication of Antibacterial TiO₂ Nanostructured Surfaces Using the Hydrothermal Method

ACS Omega. 2022 Dec 7;7(50):47070-47077. doi: 10.1021/acsomega.2c06175. eCollection 2022 Dec 20.

Authors

Niharika Rawat¹, Metka Benčina^{1

2

3}, Ekaterina Gongadze¹, Ita Junkar², Aleš Iglič^{1

4}

Affiliations

¹ Laboratory of Physics, Faculty of Electrical Engineering, University of Ljubljana, Tržaška 25, SI-1000 Ljubljana, Slovenia.
² Department of Surface Engineering, Jožef Stefan Institute, Jamova 39, SI-1000 Ljubljana, Slovenia.
³ Laboratory of Clinical Biophysics, Faculty of Health Sciences, University of Ljubljana, Zdravstvena pot 5, SI-1000 Ljubljana, Slovenia.
⁴ Chair of Orthopaedic Surgery, Faculty of Medicine, University of Ljubljana, Vrazov trg 2, SI-1000 Ljubljana, Slovenia.

Abstract

Implant-associated infections (IAI) are a common cause for implant failure, increased medical costs, and critical for patient healthcare. Infections are a result of bacterial colonization, which leads to biofilm formation on the implant surface. Nanostructured surfaces have been shown to have the potential to inhibit bacterial adhesion mainly due to antibacterial efficacy of their unique surface nanotopography. The change in topography affects the physicochemical properties of their surface such as surface chemistry, morphology, wettability, surface charge, and even electric field which influences the biological response. In this study, a conventional and cost-effective hydrothermal method was used to fabricate nanoscale protrusions of various dimensions on the surface of Ti, Ti₆Al₄V, and NiTi materials, commonly used in biomedical applications. The morphology, surface chemistry, and wettability were analyzed using scanning electron microscopy (SEM), X-ray photoemission spectroscopy (XPS), and water contact angle analysis. The antibacterial efficacy of the synthesized nanostructures was analyzed by the use of Escherichia coli bacterial strain. XPS analysis revealed that the concentration of oxygen and titanium increased on Ti and Ti₆Al₄V, which indicates that TiO₂ is formed on the surface. The concentration of oxygen and titanium however decreased on the NiTi surface after hydrothermal treatment, and also a small amount of Ni was detected. SEM analysis showed that by hydrothermal treatment alterations in the surface topography of the TiO₂ layer could be achieved. The oxide layer on the NiTi prepared by the hydrothermal method contains a low amount of Ni (2.8 atom %), which is especially important for implantable materials. The results revealed that nanostructured surfaces significantly reduced bacterial adhesion on the Ti, Ti₆Al₄V, and NiTi surface compared to the untreated surfaces used as a control. Furthermore, two sterilization techniques were also studied to evaluate the stability of the nanostructure and its influence on the antibacterial activity. Sterilization with UV light seems to more efficiently inhibit bacterial growth on the hydrothermally modified Ti₆Al₄V surface, which was further reduced for hydrothermally treated Ti and NiTi. The developed nanostructured surfaces of Ti and its alloys can pave a way for the fabrication of antibacterial surfaces that reduce the likelihood of IAI.