From DNA barcodes to ecology: Meta-analysis of central European beetles reveal link with species ecology but also to data pattern and gaps

Sara Ottati; Jonas Eberle; Björn Rulik; Frank Köhler; Dirk Ahrens

doi:10.1002/ece3.9650

From DNA barcodes to ecology: Meta-analysis of central European beetles reveal link with species ecology but also to data pattern and gaps

Ecol Evol. 2022 Dec 21;12(12):e9650. doi: 10.1002/ece3.9650. eCollection 2022 Dec.

Authors

Sara Ottati^{1

2}, Jonas Eberle^{1

3}, Björn Rulik², Frank Köhler⁴, Dirk Ahrens¹

Affiliations

¹ Zoologisches Forschungsmuseum A. Koenig (LIB) Bonn Germany.
² Department of Agricultural, Forest and Food Sciences (DISAFA) University of Torino Turin Italy.
³ Department of Environment & Biodiversity University of Salzburg Salzburg Austria.
⁴ Coleopterological Research Office Bornheim Germany.

Abstract

DNA barcoding has been used worldwide to identify biological specimens and to delimit species. It represents a cost-effective, fast, and efficient way to assess biodiversity with help of the public Barcode of Life Database (BOLD) accounting for more than 236,000 animal species and more than 10 million barcode sequences. Here, we performed a meta-analysis of available barcode data of central European Coleoptera to detect intraspecific genetic patterns among ecological groups in relation to geographic distance with the aim to investigate a possible link between infraspecific variation and species ecology. We collected information regarding feeding style, body size, as well as habitat and biotope preferences. Mantel tests and two variants of Procrustes analysis, both involving the Principal Coordinates Neighborhood Matrices (PCNM) approach, were applied on genetic and geographic distance matrices. However, significance levels were too low to further use the outcome for further trait investigation: these were in mean for all ecological guilds only 7.5, 9.4, or 15.6% for PCNM + PCA, NMDS + PCA, and Mantel test, respectively, or at best 28% for a single guild. Our study confirmed that certain ecological traits were associated with higher species diversity and foster stronger genetic differentiation. Results suggest that increased numbers of species, sampling localities, and specimens for a chosen area of interest may give new insights to explore barcode data and species ecology for the scope of conservation on a larger scale. We performed a meta-analysis of available barcode data of central European beetles to detect intraspecific genetic patterns among ecological groups in relation to geographic distance, regarding feeding style, body size, as well as habitat and biotope preferences. Our study confirmed that certain ecological traits were associated with higher species diversity and foster stronger genetic differentiation. However, significance levels were too low to further use the outcome for further trait investigation.

Keywords: Central Europe; Coleoptera; DNA barcoding; GBOL.