Trans-anethole exerts protective effects on lipopolysaccharide-induced acute jejunal inflammation of broilers via repressing NF-κB signaling pathway

Poult Sci. 2023 Feb;102(2):102397. doi: 10.1016/j.psj.2022.102397. Epub 2022 Dec 9.

Abstract

This study aimed to explore the effects of trans-anethole (TA) on lipopolysaccharide (LPS)-induced acute jejunal inflammation model of broilers. A total of 160 one-day-old broilers (male; Arbor Acres) were randomly allocated into four treatment groups with 8 replicates of 5 birds each. On d 20, the dose of 5 mg/kg body weight LPS solution and the equal amount of sterile saline were intraperitoneally injected into LPS-challenged and unchallenged broilers, respectively. Compared with the control group, LPS decreased (P < 0.05) the villus height (VH) and the ratio of villus height to crypt depth (VCR) but increased (P < 0.05) the crypt depth (CD), meanwhile, enhanced (P < 0.01) the levels of interleukin-6 (IL-6), interleukin-1beta (IL-1β) and tumor necrosis factor-alpha (TNF-α) but decreased (P < 0.01) the level of interleukin-10 (IL-10). The group supplemented with 600 mg/kg of TA had lower (P < 0.01) CD and higher (P < 0.01) VCR than the LPS group. TA increased (P < 0.01) the level of IL-10 and decreased (P < 0.01) the level of IL-1β. The mRNA expression levels of IL-6, nuclear factor kappa B (NF-κB), TNF-α were up-regulated (P < 0.05) and the levels of IL-10 and inhibitor of NF-κB alpha (IκBα) were down-regulated (P < 0.05) by LPS as compared with the control group. TA down-regulated (P < 0.05) the increased mRNA expression levels of genes caused by LPS, as well as up-regulated (P < 0.05) the levels of IL-10 and IκBα. Furthermore, LPS down-regulated (P < 0.05) and up-regulated (P < 0.05) the protein expression levels of IκBα and NF-κB p65, respectively. TA up-regulated (P < 0.05) the level of IκBα and down-regulated (P < 0.05) the level of NF-κB p65. The conclusion of this study is that TA could exert protective effect on the LPS-induced acute jejunal inflammation of broilers via repressing the activation of NF-κB and the 600 mg/kg is the optimal dose against LPS-induced acute jejunal inflammation of broilers.

Keywords: NF-κB signaling pathway; acute inflammation; broiler; intestine; lipopolysaccharide; trans-anethole.

Publication types

  • Randomized Controlled Trial, Veterinary

MeSH terms

  • Animals
  • Chickens / genetics
  • Inflammation / chemically induced
  • Inflammation / drug therapy
  • Inflammation / veterinary
  • Interleukin-10 / pharmacology
  • Interleukin-6 / metabolism
  • Lipopolysaccharides* / pharmacology
  • Male
  • NF-KappaB Inhibitor alpha / metabolism
  • NF-KappaB Inhibitor alpha / pharmacology
  • NF-KappaB Inhibitor alpha / therapeutic use
  • NF-kappa B* / metabolism
  • RNA, Messenger / genetics
  • Signal Transduction
  • Tumor Necrosis Factor-alpha

Substances

  • NF-kappa B
  • Lipopolysaccharides
  • Interleukin-10
  • NF-KappaB Inhibitor alpha
  • anethole
  • Interleukin-6
  • Tumor Necrosis Factor-alpha
  • RNA, Messenger