Utilizing chemotherapy-induced tumor RNA nanoparticles to improve cancer chemoimmunotherapy

Lanhong Su; Wen Pan; Xiangxia Li; Xingyu Zhou; Xiaopeng Ma; Yuanzeng Min

doi:10.1016/j.actbio.2022.12.039

Utilizing chemotherapy-induced tumor RNA nanoparticles to improve cancer chemoimmunotherapy

Acta Biomater. 2023 Mar 1:158:698-707. doi: 10.1016/j.actbio.2022.12.039. Epub 2022 Dec 21.

Authors

Lanhong Su¹, Wen Pan¹, Xiangxia Li¹, Xingyu Zhou¹, Xiaopeng Ma², Yuanzeng Min³

Affiliations

¹ Department of Chemistry, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China.
² The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China. Electronic address: doc_mxp@126.com.
³ Department of Chemistry, Hefei National Research Center for Physical Sciences at the Microscale, University of Science and Technology of China, Hefei, China; The First Affiliated Hospital of USTC, Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China; CAS Key Lab of Soft Matter Chemistry, University of Science and Technology of China, Hefei, China. Electronic address: minyz@ustc.edu.cn.

PMID: 36563773
DOI: 10.1016/j.actbio.2022.12.039

Abstract

Chemotherapy has become a popular combination strategy to improve the response rate of immunotherapy since certain chemotherapeutic drugs kill tumor cells by an immunogenic cell death (ICD) pathway, which activates antitumor immune responses. Unfortunately, the synergistic effect of chemoimmunotherapy can be impaired due to the toxicities of chemotherapeutic agent-induced lymphatic depletion and immunosuppression. In this study, we present an approach to improve immunotherapy by using tumor RNA nanoparticles (RNA-NPs) where RNA is directly extracted from chemotherapy-treated cancer cells and then condensed by protamine via electrostatic interactions to form complexes. Such RNA-NPs can be effectively taken up by dendritic cells (DCs) in the draining lymph nodes after subcutaneous injection. Compared with noninduced tumor RNA nanoparticles (N-RNA-NPs), chemotherapy-induced tumor RNA nanoparticles (C-RNA-NPs) can significantly promote DC maturation and stimulate a stronger immune response against established CT-26 colon carcinoma. Besides, C-RNA-NPs can improve the efficacy of immune checkpoint blockade (ICB) therapy by facilitating the infiltration of intratumoral T cells and increasing the ratio of CD8⁺ T cells to regulatory T cells (Tregs). More importantly, the synergistic effect of chemoimmunotherapy is also enhanced by treatment with C-RNA-NPs. STATEMENT OF SIGNIFICANCE: Although immune checkpoint blockade therapy has been demonstrated to be effective in some advanced cancers, the low response rate has significantly limited its clinical application. To address this issue, a new strategy for improving cancer immunotherapy using chemotherapy-induced tumor RNA nanoparticles (C-RNA-NPs) is developed in this work. The proposed C-RNA-NPs could be captured by dendritic cells, which were then stimulated to the maturation status to initiate an anticancer immune response. Furthermore, the response rate to immunotherapy was significantly increased by promoting intratumoral T-cell infiltration and elevating the intratumoral ratio of CD8⁺ T cells to regulatory T cells after treatment with C-RNA-NPs. Therefore, C-RNA-NPs have the potential to improve cancer immunotherapy.

Keywords: Chemoimmunotherapy; Chemotherapy; Immune checkpoint blockade; RNA vaccine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

CD8-Positive T-Lymphocytes
Cell Line, Tumor
Humans
Immune Checkpoint Inhibitors
Immunotherapy
Nanoparticles* / chemistry
Neoplasms* / drug therapy
RNA / pharmacology
RNA, Neoplasm
Tumor Microenvironment

Substances

Immune Checkpoint Inhibitors
RNA
RNA, Neoplasm