The aim of this study was to investigate how the freezing and thawing of biological tissues affect their complex permittivity in the microwave frequency range from 0.5 MHz to 18 GHz. We measured the complex permittivity of ex vivo bovine tissues, including brain white and grey matter, liver, and muscle, using an open-ended coaxial probe. Bovine tissues were chosen for their availability and similarity to human tissue permittivity. The samples were measured at 25 °C, before they were frozen either in a commercial freezer below -18 °C or in liquid nitrogen, nominally at -196 °C. The measured permittivity before freezing was compared to the permittivity measured after freezing and thawing the tissues back to 25 °C. Statistical analysis of the results showed a statistically significant change in permittivity after freezing and thawing by both methods for all the measured tissues, at least in some parts of the measured frequency range. The largest difference was observed for the white matter, while the liver had the smallest percent change.
Keywords: brain white and grey matter; complex permittivity measurement; ex vivo bovine tissues; freezing and thawing of biological tissues; liver; microwave frequency range; muscle; open-ended coaxial probe.