Terahertz Combined with Metamaterial Microfluidic Chip for Troponin Antigen Detection

Yen-Shuo Lin; Shih-Ting Huang; Shen-Fu Hsu; Kai-Yuan Tang; Ta-Jen Yen; Da-Jeng Yao

doi:10.3390/mi13122257

Terahertz Combined with Metamaterial Microfluidic Chip for Troponin Antigen Detection

Micromachines (Basel). 2022 Dec 19;13(12):2257. doi: 10.3390/mi13122257.

Authors

Yen-Shuo Lin¹, Shih-Ting Huang², Shen-Fu Hsu³, Kai-Yuan Tang³, Ta-Jen Yen⁴, Da-Jeng Yao¹

Affiliations

¹ Institute of NanoEngineering and MicroSystems, National Tsing Hua University, Hsinchu 30013, Taiwan.
² Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.
³ ACE BIOTEK Co., Ltd., Hsinchu 30261, Taiwan.
⁴ Department of Material Science Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan.

Abstract

In this paper, we use terahertz combined with metamaterial technology as a powerful tool to identify analytes at different concentrations. Combined with the microfluidic chip, the experimental measurement can be performed with a small amount of analyte. In detecting the troponin antigen, surface modification is carried out by biochemical binding. Through the observation of fluorescent antibodies, the average number of fluorescent dots per unit of cruciform metamaterial is 25.60, and then, by adjusting the binding temperature and soaking time, the average number of fluorescent dots per unit of cruciform metamaterial can be increased to 181.02. Through the observation of fluorescent antibodies, it is confirmed that the antibodies can be successfully stabilized on the metamaterial and then bound to the target antigen. The minimum detectable concentration is between 0.05~0.1 μg/100 μL, and the concentration and ΔY show a positive correlation of R² = 0.9909.

Keywords: antigen; metamaterials; microfluidics; terahertz; troponin.

Grants and funding

This research was funded by Ministry of Science and Technology, Taiwan, MOST 108-2622-E-007-027-CC2 and MOST 109-2622-E-007-008-CC2.